1lu3: Difference between revisions

Revision as of 14:48, 21 February 2008

Separate Fitting of the Anticodon Loop Region of tRNA (nucleotide 26-42) in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome

OverviewOverview

During the elongation cycle of protein biosynthesis, the specific amino acid coded for by the mRNA is delivered by a complex that is comprised of the cognate aminoacyl-tRNA, elongation factor Tu and GTP. As this ternary complex binds to the ribosome, the anticodon end of the tRNA reaches the decoding center in the 30S subunit. Here we present the cryo- electron microscopy (EM) study of an Escherichia coli 70S ribosome-bound ternary complex stalled with an antibiotic, kirromycin. In the cryo-EM map the anticodon arm of the tRNA presents a new conformation that appears to facilitate the initial codon-anticodon interaction. Furthermore, the elbow region of the tRNA is seen to contact the GTPase-associated center on the 50S subunit of the ribosome, suggesting an active role of the tRNA in the transmission of the signal prompting the GTP hydrolysis upon codon recognition.

About this StructureAbout this Structure

1LU3 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process., Valle M, Sengupta J, Swami NK, Grassucci RA, Burkhardt N, Nierhaus KH, Agrawal RK, Frank J, EMBO J. 2002 Jul 1;21(13):3557-67. PMID:12093756

Page seeded by OCA on Thu Feb 21 13:48:28 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1lu3.gif|left|200px]]<br /><applet load="1lu3" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1lu3.gif|left|200px]]<br /><applet load="1lu3" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1lu3, resolution 16.8&Aring;" />
 '''Separate Fitting of the Anticodon Loop Region of tRNA (nucleotide 26-42) in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome'''<br />
 ==Overview==
-During the elongation cycle of protein biosynthesis, the specific amino, acid coded for by the mRNA is delivered by a complex that is comprised of, the cognate aminoacyl-tRNA, elongation factor Tu and GTP. As this ternary, complex binds to the ribosome, the anticodon end of the tRNA reaches the, decoding center in the 30S subunit. Here we present the cryo- electron, microscopy (EM) study of an Escherichia coli 70S ribosome-bound ternary, complex stalled with an antibiotic, kirromycin. In the cryo-EM map the, anticodon arm of the tRNA presents a new conformation that appears to, facilitate the initial codon-anticodon interaction. Furthermore, the elbow, region of the tRNA is seen to contact the GTPase-associated center on the, 50S subunit of the ribosome, suggesting an active role of the tRNA in the, transmission of the signal prompting the GTP hydrolysis upon codon, recognition.
+During the elongation cycle of protein biosynthesis, the specific amino acid coded for by the mRNA is delivered by a complex that is comprised of the cognate aminoacyl-tRNA, elongation factor Tu and GTP. As this ternary complex binds to the ribosome, the anticodon end of the tRNA reaches the decoding center in the 30S subunit. Here we present the cryo- electron microscopy (EM) study of an Escherichia coli 70S ribosome-bound ternary complex stalled with an antibiotic, kirromycin. In the cryo-EM map the anticodon arm of the tRNA presents a new conformation that appears to facilitate the initial codon-anticodon interaction. Furthermore, the elbow region of the tRNA is seen to contact the GTPase-associated center on the 50S subunit of the ribosome, suggesting an active role of the tRNA in the transmission of the signal prompting the GTP hydrolysis upon codon recognition.
 ==About this Structure==
-LU3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LU3 OCA].
+LU3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LU3 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Agrawal, R.K.]]
+[[Category: Agrawal, R K.]]
 [[Category: Burkhardt, N.]]
 [[Category: Frank, J.]]
-[[Category: Grassucci, R.A.]]
+[[Category: Grassucci, R A.]]
-[[Category: Nierhaus, K.H.]]
+[[Category: Nierhaus, K H.]]
 [[Category: Sengupta, J.]]
-[[Category: Swami, N.K.]]
+[[Category: Swami, N K.]]
 [[Category: Valle, M.]]
-[[Category: 70s e.coli ribosome]]
+[[Category: 70s e coli ribosome]]
 [[Category: conformational change]]
 [[Category: cryo-em]]
@@ Line 27: / Line 27: @@
 [[Category: trna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:22:48 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:28 2008''