1lnq: Difference between revisions

Revision as of 14:46, 21 February 2008

CRYSTAL STRUCTURE OF MTHK AT 3.3 A

OverviewOverview

Ion channels exhibit two essential biophysical properties; that is, selective ion conduction, and the ability to gate-open in response to an appropriate stimulus. Two general categories of ion channel gating are defined by the initiating stimulus: ligand binding (neurotransmitter- or second-messenger-gated channels) or membrane voltage (voltage-gated channels). Here we present the structural basis of ligand gating in a K(+) channel that opens in response to intracellular Ca(2+). We have cloned, expressed, analysed electrical properties, and determined the crystal structure of a K(+) channel (MthK) from Methanobacterium thermoautotrophicum in the Ca(2+)-bound, opened state. Eight RCK domains (regulators of K(+) conductance) form a gating ring at the intracellular membrane surface. The gating ring uses the free energy of Ca(2+) binding in a simple manner to perform mechanical work to open the pore.

About this StructureAbout this Structure

1LNQ is a Single protein structure of sequence from Methanothermobacter thermautotrophicus with as ligand. The following page contains interesting information on the relation of 1LNQ with [Potassium Channels]. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure and mechanism of a calcium-gated potassium channel., Jiang Y, Lee A, Chen J, Cadene M, Chait BT, MacKinnon R, Nature. 2002 May 30;417(6888):515-22. PMID:12037559

Page seeded by OCA on Thu Feb 21 13:46:39 2008

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OCA

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-[[Image:1lnq.gif|left|200px]]<br />
+[[Image:1lnq.gif|left|200px]]<br /><applet load="1lnq" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1lnq" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1lnq, resolution 3.30&Aring;" />
 '''CRYSTAL STRUCTURE OF MTHK AT 3.3 A'''<br />
 ==Overview==
-Ion channels exhibit two essential biophysical properties; that is, selective ion conduction, and the ability to gate-open in response to an, appropriate stimulus. Two general categories of ion channel gating are, defined by the initiating stimulus: ligand binding (neurotransmitter- or, second-messenger-gated channels) or membrane voltage (voltage-gated, channels). Here we present the structural basis of ligand gating in a K(+), channel that opens in response to intracellular Ca(2+). We have cloned, expressed, analysed electrical properties, and determined the crystal, structure of a K(+) channel (MthK) from Methanobacterium, thermoautotrophicum in the Ca(2+)-bound, opened state. Eight RCK domains, (regulators of K(+) conductance) form a gating ring at the intracellular, membrane surface. The gating ring uses the free energy of Ca(2+) binding, in a simple manner to perform mechanical work to open the pore.
+Ion channels exhibit two essential biophysical properties; that is, selective ion conduction, and the ability to gate-open in response to an appropriate stimulus. Two general categories of ion channel gating are defined by the initiating stimulus: ligand binding (neurotransmitter- or second-messenger-gated channels) or membrane voltage (voltage-gated channels). Here we present the structural basis of ligand gating in a K(+) channel that opens in response to intracellular Ca(2+). We have cloned, expressed, analysed electrical properties, and determined the crystal structure of a K(+) channel (MthK) from Methanobacterium thermoautotrophicum in the Ca(2+)-bound, opened state. Eight RCK domains (regulators of K(+) conductance) form a gating ring at the intracellular membrane surface. The gating ring uses the free energy of Ca(2+) binding in a simple manner to perform mechanical work to open the pore.
 ==About this Structure==
-LNQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1LNQ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb38_1.html Potassium Channels]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LNQ OCA].
+LNQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1LNQ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb38_1.html Potassium Channels]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNQ OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Cadene, M.]]
-[[Category: Chait, B.T.]]
+[[Category: Chait, B T.]]
 [[Category: Chen, J.]]
 [[Category: Jiang, Y.]]
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 [[Category: rossman fold]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:03:20 2007''
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