1lmv: Difference between revisions
New page: left|200px<br /><applet load="1lmv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lmv" /> '''Solution structure of the unmodified U2 snRN... |
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[[Image:1lmv.jpg|left|200px]]<br /><applet load="1lmv" size=" | [[Image:1lmv.jpg|left|200px]]<br /><applet load="1lmv" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae'''<br /> | '''Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae'''<br /> | ||
==Overview== | ==Overview== | ||
Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a | Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing. | ||
==About this Structure== | ==About this Structure== | ||
1LMV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | 1LMV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Greenbaum, N | [[Category: Greenbaum, N L.]] | ||
[[Category: Newby, M | [[Category: Newby, M I.]] | ||
[[Category: a-form helix]] | [[Category: a-form helix]] | ||
[[Category: branch site]] | [[Category: branch site]] | ||
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[[Category: u2 snrna]] | [[Category: u2 snrna]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:31 2008'' | ||
