1llm: Difference between revisions
New page: left|200px<br /><applet load="1llm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1llm, resolution 1.50Å" /> '''Crystal Structure of... |
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[[Image:1llm.jpg|left|200px]]<br /><applet load="1llm" size=" | [[Image:1llm.jpg|left|200px]]<br /><applet load="1llm" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1llm, resolution 1.50Å" /> | caption="1llm, resolution 1.50Å" /> | ||
'''Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA'''<br /> | '''Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA'''<br /> | ||
==Overview== | ==Overview== | ||
Proteins that employ dimerization domains to bind cooperatively to DNA | Proteins that employ dimerization domains to bind cooperatively to DNA have a number of potential advantages over monomers with regards to gene regulation. Using a combination of structure-based design and phage display, a dimeric Cys(2)His(2) zinc finger protein has been created that binds cooperatively to DNA via an attached leucine zipper dimerization domain. This chimera, derived from components of Zif268 and GCN4, displayed excellent DNA-binding specificity, and we now report the 1.5 A resolution cocrystal structure of the Zif268-GCN4 homodimer bound to DNA. This structure shows how phage display has annealed the DNA binding and dimerization domains into a single functional unit. Moreover, this chimera provides a potential platform for the creation heterodimeric zinc finger proteins that can regulate a desired target gene through cooperative DNA recognition. | ||
==About this Structure== | ==About this Structure== | ||
1LLM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus_and_saccharomyces_cerevisiae Mus musculus and saccharomyces cerevisiae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1LLM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus_and_saccharomyces_cerevisiae Mus musculus and saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LLM OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Mus musculus and saccharomyces cerevisiae]] | [[Category: Mus musculus and saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Grant, R | [[Category: Grant, R A.]] | ||
[[Category: Pabo, C | [[Category: Pabo, C O.]] | ||
[[Category: Wolfe, S | [[Category: Wolfe, S A.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: dimerization]] | [[Category: dimerization]] | ||
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[[Category: zinc fingers]] | [[Category: zinc fingers]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:09 2008'' | ||
Revision as of 14:46, 21 February 2008
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Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA
OverviewOverview
Proteins that employ dimerization domains to bind cooperatively to DNA have a number of potential advantages over monomers with regards to gene regulation. Using a combination of structure-based design and phage display, a dimeric Cys(2)His(2) zinc finger protein has been created that binds cooperatively to DNA via an attached leucine zipper dimerization domain. This chimera, derived from components of Zif268 and GCN4, displayed excellent DNA-binding specificity, and we now report the 1.5 A resolution cocrystal structure of the Zif268-GCN4 homodimer bound to DNA. This structure shows how phage display has annealed the DNA binding and dimerization domains into a single functional unit. Moreover, this chimera provides a potential platform for the creation heterodimeric zinc finger proteins that can regulate a desired target gene through cooperative DNA recognition.
About this StructureAbout this Structure
1LLM is a Single protein structure of sequence from Mus musculus and saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a designed dimeric zinc finger protein bound to DNA., Wolfe SA, Grant RA, Pabo CO, Biochemistry. 2003 Nov 25;42(46):13401-9. PMID:14621985
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