1kv9: Difference between revisions
New page: left|200px<br /><applet load="1kv9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kv9, resolution 1.9Å" /> '''Structure at 1.9 A Re... |
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[[Image:1kv9.jpg|left|200px]]<br /><applet load="1kv9" size=" | [[Image:1kv9.jpg|left|200px]]<br /><applet load="1kv9" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1kv9, resolution 1.9Å" /> | caption="1kv9, resolution 1.9Å" /> | ||
'''Structure at 1.9 A Resolution of a Quinohemoprotein Alcohol Dehydrogenase from Pseudomonas putida HK5'''<br /> | '''Structure at 1.9 A Resolution of a Quinohemoprotein Alcohol Dehydrogenase from Pseudomonas putida HK5'''<br /> | ||
==Overview== | ==Overview== | ||
The type II quinohemoprotein alcohol dehydrogenase of Pseudomonas putida | The type II quinohemoprotein alcohol dehydrogenase of Pseudomonas putida is a periplasmic enzyme that oxidizes substrate alcohols to the aldehyde and transfers electrons first to pyrroloquinoline quinone (PQQ) and then to an internal heme group. The 1.9 A resolution crystal structure reveals that the enzyme contains a large N-terminal eight-stranded beta propeller domain (approximately 60 kDa) similar to methanol dehydrogenase and a small C-terminal c-type cytochrome domain (approximately 10 kDa) similar to the cytochrome subunit of p-cresol methylhydoxylase. The PQQ is bound near the axis of the propeller domain about 14 A from the heme. A molecule of acetone, the product of the oxidation of isopropanol present during crystallization, appears to be bound in the active site cavity. | ||
==About this Structure== | ==About this Structure== | ||
1KV9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with CA, PQQ, HEM, EPE, ACN and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1KV9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=PQQ:'>PQQ</scene>, <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=EPE:'>EPE</scene>, <scene name='pdbligand=ACN:'>ACN</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KV9 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Adachi, O.]] | [[Category: Adachi, O.]] | ||
[[Category: Bellamy, H | [[Category: Bellamy, H D.]] | ||
[[Category: Chen, Z | [[Category: Chen, Z W.]] | ||
[[Category: Fujii, T.]] | [[Category: Fujii, T.]] | ||
[[Category: Mathews, F | [[Category: Mathews, F S.]] | ||
[[Category: Matsushita, K.]] | [[Category: Matsushita, K.]] | ||
[[Category: Toyama, H.]] | [[Category: Toyama, H.]] | ||
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[[Category: quinohemoprotein alcohol dehydrogenase]] | [[Category: quinohemoprotein alcohol dehydrogenase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:38:15 2008'' | ||
Revision as of 14:38, 21 February 2008
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Structure at 1.9 A Resolution of a Quinohemoprotein Alcohol Dehydrogenase from Pseudomonas putida HK5
OverviewOverview
The type II quinohemoprotein alcohol dehydrogenase of Pseudomonas putida is a periplasmic enzyme that oxidizes substrate alcohols to the aldehyde and transfers electrons first to pyrroloquinoline quinone (PQQ) and then to an internal heme group. The 1.9 A resolution crystal structure reveals that the enzyme contains a large N-terminal eight-stranded beta propeller domain (approximately 60 kDa) similar to methanol dehydrogenase and a small C-terminal c-type cytochrome domain (approximately 10 kDa) similar to the cytochrome subunit of p-cresol methylhydoxylase. The PQQ is bound near the axis of the propeller domain about 14 A from the heme. A molecule of acetone, the product of the oxidation of isopropanol present during crystallization, appears to be bound in the active site cavity.
About this StructureAbout this Structure
1KV9 is a Single protein structure of sequence from Pseudomonas putida with , , , , and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Structure at 1.9 A resolution of a quinohemoprotein alcohol dehydrogenase from Pseudomonas putida HK5., Chen ZW, Matsushita K, Yamashita T, Fujii TA, Toyama H, Adachi O, Bellamy HD, Mathews FS, Structure. 2002 Jun;10(6):837-49. PMID:12057198
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