1kog: Difference between revisions
New page: left|200px<br /><applet load="1kog" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kog, resolution 3.50Å" /> '''Crystal structure of... |
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[[Image:1kog.gif|left|200px]]<br /><applet load="1kog" size=" | [[Image:1kog.gif|left|200px]]<br /><applet load="1kog" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1kog, resolution 3.50Å" /> | caption="1kog, resolution 3.50Å" /> | ||
'''Crystal structure of E. coli threonyl-tRNA synthetase interacting with the essential domain of its mRNA operator'''<br /> | '''Crystal structure of E. coli threonyl-tRNA synthetase interacting with the essential domain of its mRNA operator'''<br /> | ||
==Overview== | ==Overview== | ||
Escherichia coli threonyl-tRNA synthetase (ThrRS) represses the | Escherichia coli threonyl-tRNA synthetase (ThrRS) represses the translation of its own messenger RNA by binding to an operator located upstream of the initiation codon. The crystal structure of the complex between the core of ThrRS and the essential domain of the operator shows that the mRNA uses the recognition mode of the tRNA anticodon loop to initiate binding. The final positioning of the operator, upon which the control mechanism is based, relies on a characteristic RNA motif adapted to the enzyme surface. The finding of other thrS operators that have this conserved motif leads to a generalization of this regulatory mechanism to a subset of Gram-negative bacteria. | ||
==About this Structure== | ==About this Structure== | ||
1KOG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and TSB as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Threonine--tRNA_ligase Threonine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.3 6.1.1.3] Full crystallographic information is available from [http:// | 1KOG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=TSB:'>TSB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Threonine--tRNA_ligase Threonine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.3 6.1.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOG OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Threonine--tRNA ligase]] | [[Category: Threonine--tRNA ligase]] | ||
[[Category: Caillet, J.]] | [[Category: Caillet, J.]] | ||
[[Category: Dock-Bregeon, A | [[Category: Dock-Bregeon, A C.]] | ||
[[Category: Ehresmann, B.]] | [[Category: Ehresmann, B.]] | ||
[[Category: Ehresmann, C.]] | [[Category: Ehresmann, C.]] | ||
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[[Category: rna stem-loop]] | [[Category: rna stem-loop]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:17 2008'' | ||
Revision as of 14:36, 21 February 2008
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Crystal structure of E. coli threonyl-tRNA synthetase interacting with the essential domain of its mRNA operator
OverviewOverview
Escherichia coli threonyl-tRNA synthetase (ThrRS) represses the translation of its own messenger RNA by binding to an operator located upstream of the initiation codon. The crystal structure of the complex between the core of ThrRS and the essential domain of the operator shows that the mRNA uses the recognition mode of the tRNA anticodon loop to initiate binding. The final positioning of the operator, upon which the control mechanism is based, relies on a characteristic RNA motif adapted to the enzyme surface. The finding of other thrS operators that have this conserved motif leads to a generalization of this regulatory mechanism to a subset of Gram-negative bacteria.
About this StructureAbout this Structure
1KOG is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as Threonine--tRNA ligase, with EC number 6.1.1.3 Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of translational control by Escherichia coli threonyl tRNA synthetase., Torres-Larios A, Dock-Bregeon AC, Romby P, Rees B, Sankaranarayanan R, Caillet J, Springer M, Ehresmann C, Ehresmann B, Moras D, Nat Struct Biol. 2002 May;9(5):343-7. PMID:11953757
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