1kjw: Difference between revisions
New page: left|200px<br /><applet load="1kjw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kjw, resolution 1.8Å" /> '''SH3-Guanylate Kinase ... |
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[[Image:1kjw.jpg|left|200px]]<br /><applet load="1kjw" size=" | [[Image:1kjw.jpg|left|200px]]<br /><applet load="1kjw" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1kjw, resolution 1.8Å" /> | caption="1kjw, resolution 1.8Å" /> | ||
'''SH3-Guanylate Kinase Module from PSD-95'''<br /> | '''SH3-Guanylate Kinase Module from PSD-95'''<br /> | ||
==Overview== | ==Overview== | ||
Membrane-associated guanylate kinases (MAGUKs), such as PSD-95, are | Membrane-associated guanylate kinases (MAGUKs), such as PSD-95, are modular scaffolds that organize signaling complexes at synapses and other cell junctions. MAGUKs contain PDZ domains, which recruit signaling proteins, as well as a Src homology 3 (SH3) and a guanylate kinase-like (GK) domain, implicated in scaffold oligomerization. The crystal structure of the SH3-GK module from PSD-95 reveals that these domains form an integrated unit: the SH3 fold comprises noncontiguous sequence elements divided by a hinge region and the GK domain. These elements compose two subdomains that can assemble in either an intra- or intermolecular fashion to complete the SH3 fold. We propose a model for MAGUK oligomerization in which complementary SH3 subdomains associate by 3D domain swapping. This model provides a possible mechanism for ligand regulation of oligomerization. | ||
==About this Structure== | ==About this Structure== | ||
1KJW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1KJW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJW OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bredt, D | [[Category: Bredt, D S.]] | ||
[[Category: Dakoji, S | [[Category: Dakoji, S R.]] | ||
[[Category: Lim, W | [[Category: Lim, W A.]] | ||
[[Category: McGee, A | [[Category: McGee, A W.]] | ||
[[Category: Olsen, O.]] | [[Category: Olsen, O.]] | ||
[[Category: Prehoda, K | [[Category: Prehoda, K E.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: protein-protein interaction]] | [[Category: protein-protein interaction]] | ||
[[Category: scaffold]] | [[Category: scaffold]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:56 2008'' | ||
Revision as of 14:34, 21 February 2008
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SH3-Guanylate Kinase Module from PSD-95
OverviewOverview
Membrane-associated guanylate kinases (MAGUKs), such as PSD-95, are modular scaffolds that organize signaling complexes at synapses and other cell junctions. MAGUKs contain PDZ domains, which recruit signaling proteins, as well as a Src homology 3 (SH3) and a guanylate kinase-like (GK) domain, implicated in scaffold oligomerization. The crystal structure of the SH3-GK module from PSD-95 reveals that these domains form an integrated unit: the SH3 fold comprises noncontiguous sequence elements divided by a hinge region and the GK domain. These elements compose two subdomains that can assemble in either an intra- or intermolecular fashion to complete the SH3 fold. We propose a model for MAGUK oligomerization in which complementary SH3 subdomains associate by 3D domain swapping. This model provides a possible mechanism for ligand regulation of oligomerization.
About this StructureAbout this Structure
1KJW is a Single protein structure of sequence from Rattus norvegicus with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the SH3-guanylate kinase module from PSD-95 suggests a mechanism for regulated assembly of MAGUK scaffolding proteins., McGee AW, Dakoji SR, Olsen O, Bredt DS, Lim WA, Prehoda KE, Mol Cell. 2001 Dec;8(6):1291-301. PMID:11779504
Page seeded by OCA on Thu Feb 21 13:34:56 2008