1khd: Difference between revisions

Revision as of 14:34, 21 February 2008

Crystal Structure Analysis of the anthranilate phosphoribosyltransferase from Erwinia carotovora at 1.9 resolution (current name, Pectobacterium carotovorum)

OverviewOverview

The structure of anthranilate phosphoribosyltransferase from the enterobacterium Pectobacterium carotovorum has been solved at 2.4 A in complex with Mn(2+)-pyrophosphate, and at 1.9 A without ligands. The enzyme structure has a novel phosphoribosyltransferase (PRT) fold and displays close homology to the structures of pyrimidine nucleoside phosphorylases. The enzyme is a homodimer with a monomer of 345 residues. Each monomer consists of two subdomains, alpha and alpha/beta, which form a cleft containing the active site. The nature of the active site is inferred from the trapped MnPPi complex and detailed knowledge of the active sites of nucleoside phosphorylases. With the anthranilate (An)PRT structure solved, the structures of all the enzymes required for tryptophan biosynthesis are now known.

About this StructureAbout this Structure

1KHD is a Single protein structure of sequence from Pectobacterium carotovorum. Active as Anthranilate phosphoribosyltransferase, with EC number 2.4.2.18 Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of anthranilate phosphoribosyltransferase from the enterobacterium Pectobacterium carotovorum., Kim C, Xuong NH, Edwards S, Madhusudan, Yee MC, Spraggon G, Mills SE, FEBS Lett. 2002 Jul 17;523(1-3):239-46. PMID:12123839

Page seeded by OCA on Thu Feb 21 13:34:10 2008

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OCA

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-[[Image:1khd.jpg|left|200px]]<br /><applet load="1khd" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1khd.jpg|left|200px]]<br /><applet load="1khd" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1khd, resolution 1.86&Aring;" />
 '''Crystal Structure Analysis of the anthranilate phosphoribosyltransferase from Erwinia carotovora at 1.9 resolution (current name, Pectobacterium carotovorum)'''<br />
 ==Overview==
-The structure of anthranilate phosphoribosyltransferase from the, enterobacterium Pectobacterium carotovorum has been solved at 2.4 A in, complex with Mn(2+)-pyrophosphate, and at 1.9 A without ligands. The, enzyme structure has a novel phosphoribosyltransferase (PRT) fold and, displays close homology to the structures of pyrimidine nucleoside, phosphorylases. The enzyme is a homodimer with a monomer of 345 residues., Each monomer consists of two subdomains, alpha and alpha/beta, which form, a cleft containing the active site. The nature of the active site is, inferred from the trapped MnPPi complex and detailed knowledge of the, active sites of nucleoside phosphorylases. With the anthranilate (An)PRT, structure solved, the structures of all the enzymes required for, tryptophan biosynthesis are now known.
+The structure of anthranilate phosphoribosyltransferase from the enterobacterium Pectobacterium carotovorum has been solved at 2.4 A in complex with Mn(2+)-pyrophosphate, and at 1.9 A without ligands. The enzyme structure has a novel phosphoribosyltransferase (PRT) fold and displays close homology to the structures of pyrimidine nucleoside phosphorylases. The enzyme is a homodimer with a monomer of 345 residues. Each monomer consists of two subdomains, alpha and alpha/beta, which form a cleft containing the active site. The nature of the active site is inferred from the trapped MnPPi complex and detailed knowledge of the active sites of nucleoside phosphorylases. With the anthranilate (An)PRT structure solved, the structures of all the enzymes required for tryptophan biosynthesis are now known.
 ==About this Structure==
-KHD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Active as [http://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KHD OCA].
+KHD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Active as [http://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHD OCA].
 ==Reference==
@@ Line 17: / Line 17: @@
 [[Category: Kim, C.]]
 [[Category: Madhusudan]]
-[[Category: Mills, S.E.]]
+[[Category: Mills, S E.]]
 [[Category: Spraggon, G.]]
-[[Category: Xuong, N.H.]]
+[[Category: Xuong, N H.]]
-[[Category: Yee, M.C.]]
+[[Category: Yee, M C.]]
 [[Category: nucleotide binding fold]]
 [[Category: type 3 prt fold]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:22:25 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:10 2008''