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Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b

OverviewOverview

The PWWP domain is a weakly conserved sequence motif found in > 60 eukaryotic proteins, including the mammalian DNA methyltransferases Dnmt3a and Dnmt3b. These proteins often contain other chromatin-association domains. A 135-residue PWWP domain from mouse Dnmt3b (amino acids 223--357) has been structurally characterized at 1.8 A resolution. The N-terminal half of this domain resembles a barrel-like five-stranded structure, whereas the C-terminal half contains a five-helix bundle. The two halves are packed against each other to form a single structural module that exhibits a prominent positive electrostatic potential. The PWWP domain alone binds DNA in vitro, probably through its basic surface. We also show that recombinant Dnmt3b2 protein (a splice variant of Dnmt3b) and two N-terminal deletion mutants (Delta218 and Delta369) have approximately equal methyl transfer activity on unmethylated and hemimethylated CpG-containing oligonucleotides. The Delta218 protein, which includes the PWWP domain, binds DNA more strongly than Delta369, which lacks the PWWP domain.

About this StructureAbout this Structure

1KHC is a Single protein structure of sequence from Mus musculus with as ligand. Active as DNA (cytosine-5-)-methyltransferase, with EC number 2.1.1.37 Full crystallographic information is available from OCA.

ReferenceReference

The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds., Qiu C, Sawada K, Zhang X, Cheng X, Nat Struct Biol. 2002 Mar;9(3):217-24. PMID:11836534

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 '''Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b'''<br />
 ==Overview==
-The PWWP domain is a weakly conserved sequence motif found in &gt; 60, eukaryotic proteins, including the mammalian DNA methyltransferases Dnmt3a, and Dnmt3b. These proteins often contain other chromatin-association, domains. A 135-residue PWWP domain from mouse Dnmt3b (amino acids, 223--357) has been structurally characterized at 1.8 A resolution. The, N-terminal half of this domain resembles a barrel-like five-stranded, structure, whereas the C-terminal half contains a five-helix bundle. The, two halves are packed against each other to form a single structural, module that exhibits a prominent positive electrostatic potential. The, PWWP domain alone binds DNA in vitro, probably through its basic surface., We also show that recombinant Dnmt3b2 protein (a splice variant of Dnmt3b), and two N-terminal deletion mutants (Delta218 and Delta369) have, approximately equal methyl transfer activity on unmethylated and, hemimethylated CpG-containing oligonucleotides. The Delta218 protein, which includes the PWWP domain, binds DNA more strongly than Delta369, which lacks the PWWP domain.
+The PWWP domain is a weakly conserved sequence motif found in &gt; 60 eukaryotic proteins, including the mammalian DNA methyltransferases Dnmt3a and Dnmt3b. These proteins often contain other chromatin-association domains. A 135-residue PWWP domain from mouse Dnmt3b (amino acids 223--357) has been structurally characterized at 1.8 A resolution. The N-terminal half of this domain resembles a barrel-like five-stranded structure, whereas the C-terminal half contains a five-helix bundle. The two halves are packed against each other to form a single structural module that exhibits a prominent positive electrostatic potential. The PWWP domain alone binds DNA in vitro, probably through its basic surface. We also show that recombinant Dnmt3b2 protein (a splice variant of Dnmt3b) and two N-terminal deletion mutants (Delta218 and Delta369) have approximately equal methyl transfer activity on unmethylated and hemimethylated CpG-containing oligonucleotides. The Delta218 protein, which includes the PWWP domain, binds DNA more strongly than Delta369, which lacks the PWWP domain.
 ==About this Structure==
-KHC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with UNX as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KHC OCA].
+KHC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=UNX:'>UNX</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHC OCA].
 ==Reference==
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 [[Category: five beta-sheets barrel followed by five-helix bundle]]
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