1khb: Difference between revisions
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==Overview== | ==Overview== | ||
We report crystal structures of the human enzyme phosphoenolpyruvate | We report crystal structures of the human enzyme phosphoenolpyruvate carboxykinase (PEPCK) with and without bound substrates. These structures are the first to be determined for a GTP-dependent PEPCK, and provide the first view of a novel GTP-binding site unique to the GTP-dependent PEPCK family. Three phenylalanine residues form the walls of the guanine-binding pocket on the enzyme's surface and, most surprisingly, one of the phenylalanine side-chains contributes to the enzyme's specificity for GTP. PEPCK catalyzes the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Because the gluconeogenic pathway contributes to the fasting hyperglycemia of type II diabetes, inhibitors of PEPCK may be useful in the treatment of diabetes. | ||
==Disease== | ==Disease== | ||
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[[Category: Levin, W.]] | [[Category: Levin, W.]] | ||
[[Category: Michel, H.]] | [[Category: Michel, H.]] | ||
[[Category: Ramsey, G | [[Category: Ramsey, G B.]] | ||
[[Category: Swain, A.]] | [[Category: Swain, A.]] | ||
[[Category: Weber, D.]] | [[Category: Weber, D.]] | ||
[[Category: Wertheimer, S | [[Category: Wertheimer, S J.]] | ||
[[Category: ACT]] | [[Category: ACT]] | ||
[[Category: EDO]] | [[Category: EDO]] | ||
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[[Category: p-loop]] | [[Category: p-loop]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:09 2008'' | ||
Revision as of 14:34, 21 February 2008
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PEPCK complex with nonhydrolyzable GTP analog, native data
OverviewOverview
We report crystal structures of the human enzyme phosphoenolpyruvate carboxykinase (PEPCK) with and without bound substrates. These structures are the first to be determined for a GTP-dependent PEPCK, and provide the first view of a novel GTP-binding site unique to the GTP-dependent PEPCK family. Three phenylalanine residues form the walls of the guanine-binding pocket on the enzyme's surface and, most surprisingly, one of the phenylalanine side-chains contributes to the enzyme's specificity for GTP. PEPCK catalyzes the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Because the gluconeogenic pathway contributes to the fasting hyperglycemia of type II diabetes, inhibitors of PEPCK may be useful in the treatment of diabetes.
DiseaseDisease
Known disease associated with this structure: Hypoglycemia due to PCK1 deficiency (1) OMIM:[261680]
About this StructureAbout this Structure
1KHB is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Active as Phosphoenolpyruvate carboxykinase (GTP), with EC number 4.1.1.32 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site., Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ, J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:11851336
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