1kh6: Difference between revisions

Revision as of 14:34, 21 February 2008

Crystal Structure of an RNA Tertiary Domain Essential to HCV IRES-mediated Translation Initiation.

OverviewOverview

The hepatitis C virus (HCV) internal ribosome entry site (IRES) RNA drives internal initiation of viral protein synthesis during host cell infection. In the tertiary structure of the IRES RNA, two helical junctions create recognition sites for direct binding of the 40S ribosomal subunit and eukaryotic initiation factor 3 (eIF3). The 2.8 A resolution structure of the IIIabc four-way junction, which is critical for binding eIF3, reveals how junction nucleotides interact with an adjacent helix to position regions directly involved in eIF3 recognition. Two of the emergent helices stack to form a nearly continuous A-form duplex, while stacking of the other two helices is interrupted by the insertion of junction residues into the helix minor groove. This distorted stack probably serves as an important recognition surface for the translational machinery.

About this StructureAbout this Structure

1KH6 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of an RNA tertiary domain essential to HCV IRES-mediated translation initiation., Kieft JS, Zhou K, Grech A, Jubin R, Doudna JA, Nat Struct Biol. 2002 May;9(5):370-4. PMID:11927953

Page seeded by OCA on Thu Feb 21 13:34:05 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1kh6.gif|left|200px]]<br /><applet load="1kh6" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1kh6.gif|left|200px]]<br /><applet load="1kh6" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1kh6, resolution 2.9&Aring;" />
 '''Crystal Structure of an RNA Tertiary Domain Essential to HCV IRES-mediated Translation Initiation.'''<br />
 ==Overview==
-The hepatitis C virus (HCV) internal ribosome entry site (IRES) RNA drives, internal initiation of viral protein synthesis during host cell infection., In the tertiary structure of the IRES RNA, two helical junctions create, recognition sites for direct binding of the 40S ribosomal subunit and, eukaryotic initiation factor 3 (eIF3). The 2.8 A resolution structure of, the IIIabc four-way junction, which is critical for binding eIF3, reveals, how junction nucleotides interact with an adjacent helix to position, regions directly involved in eIF3 recognition. Two of the emergent helices, stack to form a nearly continuous A-form duplex, while stacking of the, other two helices is interrupted by the insertion of junction residues, into the helix minor groove. This distorted stack probably serves as an, important recognition surface for the translational machinery.
+The hepatitis C virus (HCV) internal ribosome entry site (IRES) RNA drives internal initiation of viral protein synthesis during host cell infection. In the tertiary structure of the IRES RNA, two helical junctions create recognition sites for direct binding of the 40S ribosomal subunit and eukaryotic initiation factor 3 (eIF3). The 2.8 A resolution structure of the IIIabc four-way junction, which is critical for binding eIF3, reveals how junction nucleotides interact with an adjacent helix to position regions directly involved in eIF3 recognition. Two of the emergent helices stack to form a nearly continuous A-form duplex, while stacking of the other two helices is interrupted by the insertion of junction residues into the helix minor groove. This distorted stack probably serves as an important recognition surface for the translational machinery.
 ==About this Structure==
-KH6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KH6 OCA].
+KH6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KH6 OCA].
 ==Reference==
 Crystal structure of an RNA tertiary domain essential to HCV IRES-mediated translation initiation., Kieft JS, Zhou K, Grech A, Jubin R, Doudna JA, Nat Struct Biol. 2002 May;9(5):370-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11927953 11927953]
 [[Category: Protein complex]]
-[[Category: Doudna, J.A.]]
+[[Category: Doudna, J A.]]
 [[Category: Grech, A.]]
 [[Category: Jubin, R]]
-[[Category: Kieft, J.S.]]
+[[Category: Kieft, J S.]]
 [[Category: Zhou, K.]]
 [[Category: bromine]]
@@ Line 24: / Line 24: @@
 [[Category: translation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:21:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:05 2008''