1k6d: Difference between revisions

Revision as of 14:30, 21 February 2008

CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT

OverviewOverview

The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (>3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.

About this StructureAbout this Structure

1K6D is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Acetate CoA-transferase, with EC number 2.8.3.8 Full crystallographic information is available from OCA.

ReferenceReference

Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data., Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2116-21. Epub 2002, Nov 23. PMID:12454473

Page seeded by OCA on Thu Feb 21 13:30:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1k6d.gif|left|200px]]<br /><applet load="1k6d" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1k6d.gif|left|200px]]<br /><applet load="1k6d" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1k6d, resolution 1.90&Aring;" />
 '''CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT'''<br />
 ==Overview==
-The automation of protein structure determination is an essential, component for high-throughput structural analysis in protein X-ray, crystallography and is a key element in structural genomics. This highly, challenging undertaking relies at present on the availability of, high-quality native and derivatized protein crystals diffracting to high, or moderate resolution, respectively. Obtaining such crystals often, requires significant effort. The present study demonstrates that phases, obtained at low resolution (&gt;3.0 A) from crystals of SeMet-labeled protein, can be successfully used for automated structure determination. The, crystal structure of acetate CoA-transferase alpha-subunit was solved, using 3.4 A multi-wavelength anomalous dispersion data collected from a, crystal containing SeMet-substituted protein and 1.9 A data collected from, a native protein crystal.
+The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (&gt;3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.
 ==About this Structure==
-K6D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acetate_CoA-transferase Acetate CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.8 2.8.3.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K6D OCA].
+K6D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acetate_CoA-transferase Acetate CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.8 2.8.3.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6D OCA].
 ==Reference==
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 [[Category: Korolev, S.]]
 [[Category: Koroleva, O.]]
-[[Category: MCSG, Midwest.Center.for.Structural.Genomics.]]
+[[Category: MCSG, Midwest Center for Structural Genomics.]]
 [[Category: Petterson, K.]]
 [[Category: MG]]
@@ Line 28: / Line 28: @@
 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:53:16 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:36 2008''