1k5w: Difference between revisions

Revision as of 14:30, 21 February 2008

THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTAGMIN 1 C2B-DOMAIN: SYNAPTOTAGMIN 1 AS A PHOSPHOLIPID BINDING MACHINE

OverviewOverview

Synaptotagmin 1 probably functions as a Ca2+ sensor in neurotransmitter release via its two C2-domains, but no common Ca2+-dependent activity that could underlie a cooperative action between them has been described. The NMR structure of the C2B-domain now reveals a beta sandwich that exhibits striking similarities and differences with the C2A-domain. Whereas the bottom face of the C2B-domain has two additional alpha helices that may be involved in specialized Ca2+-independent functions, the top face binds two Ca2+ ions and is remarkably similar to the C2A-domain. Consistent with these results, but in contrast to previous studies, we find that the C2B-domain binds phospholipids in a Ca2+-dependent manner similarly to the C2A-domain. These results suggest a novel view of synaptotagmin function whereby the two C2-domains cooperate in a common activity, Ca2+-dependent phospholipid binding, to trigger neurotransmitter release.

About this StructureAbout this Structure

1K5W is a Single protein structure of sequence from Rattus norvegicus with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine., Fernandez I, Arac D, Ubach J, Gerber SH, Shin O, Gao Y, Anderson RG, Sudhof TC, Rizo J, Neuron. 2001 Dec 20;32(6):1057-69. PMID:11754837

Page seeded by OCA on Thu Feb 21 13:30:31 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1k5w.jpg|left|200px]]<br /><applet load="1k5w" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1k5w.jpg|left|200px]]<br /><applet load="1k5w" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1k5w" />
 '''THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTAGMIN 1 C2B-DOMAIN: SYNAPTOTAGMIN 1 AS A PHOSPHOLIPID BINDING MACHINE'''<br />
 ==Overview==
-Synaptotagmin 1 probably functions as a Ca2+ sensor in neurotransmitter, release via its two C2-domains, but no common Ca2+-dependent activity that, could underlie a cooperative action between them has been described. The, NMR structure of the C2B-domain now reveals a beta sandwich that exhibits, striking similarities and differences with the C2A-domain. Whereas the, bottom face of the C2B-domain has two additional alpha helices that may be, involved in specialized Ca2+-independent functions, the top face binds two, Ca2+ ions and is remarkably similar to the C2A-domain. Consistent with, these results, but in contrast to previous studies, we find that the, C2B-domain binds phospholipids in a Ca2+-dependent manner similarly to the, C2A-domain. These results suggest a novel view of synaptotagmin function, whereby the two C2-domains cooperate in a common activity, Ca2+-dependent, phospholipid binding, to trigger neurotransmitter release.
+Synaptotagmin 1 probably functions as a Ca2+ sensor in neurotransmitter release via its two C2-domains, but no common Ca2+-dependent activity that could underlie a cooperative action between them has been described. The NMR structure of the C2B-domain now reveals a beta sandwich that exhibits striking similarities and differences with the C2A-domain. Whereas the bottom face of the C2B-domain has two additional alpha helices that may be involved in specialized Ca2+-independent functions, the top face binds two Ca2+ ions and is remarkably similar to the C2A-domain. Consistent with these results, but in contrast to previous studies, we find that the C2B-domain binds phospholipids in a Ca2+-dependent manner similarly to the C2A-domain. These results suggest a novel view of synaptotagmin function whereby the two C2-domains cooperate in a common activity, Ca2+-dependent phospholipid binding, to trigger neurotransmitter release.
 ==About this Structure==
-K5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K5W OCA].
+K5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5W OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Rattus norvegicus]]
 [[Category: Single protein]]
-[[Category: Anderson, R.G.W.]]
+[[Category: Anderson, R G.W.]]
 [[Category: Arac, D.]]
 [[Category: Fernandez, I.]]
 [[Category: Gao, Y.]]
-[[Category: Gerber, S.H.]]
+[[Category: Gerber, S H.]]
 [[Category: Rizo, J.]]
 [[Category: Shin, O.]]
-[[Category: Sudhof, T.C.]]
+[[Category: Sudhof, T C.]]
 [[Category: Ubach, J.]]
 [[Category: CA]]
@@ Line 32: / Line 32: @@
 [[Category: synaptotagmin i]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:52:56 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:31 2008''