1bn7: Difference between revisions
New page: left|200px<br /> <applet load="1bn7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bn7, resolution 1.50Å" /> '''HALOALKANE DEHALOGE... |
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==About this Structure== | ==About this Structure== | ||
1BN7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]] with ACT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BN7 OCA]]. | 1BN7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]] with ACT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BN7 OCA]]. | ||
==Reference== | ==Reference== | ||
Haloalkane dehalogenases: structure of a Rhodococcus enzyme., Newman J, Peat TS, Richard R, Kan L, Swanson PE, Affholter JA, Holmes IH, Schindler JF, Unkefer CJ, Terwilliger TC, Biochemistry. 1999 Dec 7;38(49):16105-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10587433 10587433] | Haloalkane dehalogenases: structure of a Rhodococcus enzyme., Newman J, Peat TS, Richard R, Kan L, Swanson PE, Affholter JA, Holmes IH, Schindler JF, Unkefer CJ, Terwilliger TC, Biochemistry. 1999 Dec 7;38(49):16105-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10587433 10587433] | ||
[[Category: Haloalkane dehalogenase]] | |||
[[Category: Rhodococcus sp.]] | [[Category: Rhodococcus sp.]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: dhla]] | [[Category: dhla]] | ||
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Revision as of 15:23, 30 October 2007
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HALOALKANE DEHALOGENASE FROM A RHODOCOCCUS SPECIES
OverviewOverview
The hydrolytic haloalkane dehalogenases are promising bioremediation and, biocatalytic agents. Two general classes of dehalogenases have been, reported from Xanthobacter and Rhodococcus. While these enzymes share 30%, amino acid sequence identity, they have significantly different substrate, specificities and halide-binding properties. We report the 1.5 A, resolution crystal structure of the Rhodococcus dehalogenase at pH 5.5, pH, 7.0, and pH 5.5 in the presence of NaI. The Rhodococcus and Xanthobacter, enzymes have significant structural homology in the alpha/beta hydrolase, core, but differ considerably in the cap domain. Consistent with its broad, specificity for primary, secondary, and cyclic haloalkanes, the, Rhodococcus enzyme has a substantially larger active site cavity., ... [(full description)]
About this StructureAbout this Structure
1BN7 is a [Single protein] structure of sequence from [Rhodococcus sp.] with ACT as [ligand]. Active as [Haloalkane dehalogenase], with EC number [3.8.1.5]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
ReferenceReference
Haloalkane dehalogenases: structure of a Rhodococcus enzyme., Newman J, Peat TS, Richard R, Kan L, Swanson PE, Affholter JA, Holmes IH, Schindler JF, Unkefer CJ, Terwilliger TC, Biochemistry. 1999 Dec 7;38(49):16105-14. PMID:10587433
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