2j8f: Difference between revisions
New page: left|200px<br /> <applet load="2j8f" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j8f, resolution 1.84Å" /> '''CRYSTAL STRUCTURE O... |
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==About this Structure== | ==About this Structure== | ||
2J8F is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacteriophage_cp-1 Bacteriophage cp-1]] with NAG, ALA, AMV, DGL and FMT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J8F OCA]]. | 2J8F is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacteriophage_cp-1 Bacteriophage cp-1]] with NAG, ALA, AMV, DGL and FMT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Lysozyme Lysozyme]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J8F OCA]]. | ||
==Reference== | ==Reference== | ||
Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1., Perez-Dorado I, Campillo NE, Monterroso B, Hesek D, Lee M, Paez JA, Garcia P, Martinez-Ripoll M, Garcia JL, Mobashery S, Menendez M, Hermoso JA, J Biol Chem. 2007 Jun 19;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17581815 17581815] | Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1., Perez-Dorado I, Campillo NE, Monterroso B, Hesek D, Lee M, Paez JA, Garcia P, Martinez-Ripoll M, Garcia JL, Mobashery S, Menendez M, Hermoso JA, J Biol Chem. 2007 Jun 19;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17581815 17581815] | ||
[[Category: Bacteriophage cp-1]] | [[Category: Bacteriophage cp-1]] | ||
[[Category: Lysozyme]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Hermoso, J.A.]] | [[Category: Hermoso, J.A.]] | ||
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[[Category: pneumococcal cell wall degradation]] | [[Category: pneumococcal cell wall degradation]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:28:28 2007'' | ||
Revision as of 15:23, 30 October 2007
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CRYSTAL STRUCTURE OF THE MODULAR CPL-1 ENDOLYSIN COMPLEXED WITH A PEPTIDOGLYCAN ANALOGUE (E94Q MUTANT IN COMPLEX WITH A DISACCHARIDE-PENTAPEPTIDE)
OverviewOverview
Pneumococcal bacteriophage-encoded lysins are modular proteins that have, been shown to act as enzymatic antimicrobial agents (enzybiotics) in, treatment of streptococcal infections. The first X-ray crystal structures, of the Cpl-1 lysin, encoded by the pneumococcal phage Cp-1, in complex, with three bacterial cell wall peptidoglycan (PG) analogues are reported, herein. The Cpl-1 structure is folded in two well-defined modules, one, responsible for anchoring to the pneumococcal cell wall and the other, a, catalytic module, that hydrolyzes the PG. Conformational rearrangement of, Tyr127 is a critical event in molecular recognition of a stretch of five, saccharide rings of the polymeric peptidoglycan (cell wall). The PG is, bound at a stretch of the surface that is defined as the, ... [(full description)]
About this StructureAbout this Structure
2J8F is a [Single protein] structure of sequence from [Bacteriophage cp-1] with NAG, ALA, AMV, DGL and FMT as [ligands]. Active as [Lysozyme], with EC number [3.2.1.17]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1., Perez-Dorado I, Campillo NE, Monterroso B, Hesek D, Lee M, Paez JA, Garcia P, Martinez-Ripoll M, Garcia JL, Mobashery S, Menendez M, Hermoso JA, J Biol Chem. 2007 Jun 19;. PMID:17581815
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