1jwc: Difference between revisions

Revision as of 14:27, 21 February 2008

NMR SOLUTION STRUCTURE OF THE RNA HAIRPIN BINDING SITE FOR THE HISTONE STEM-LOOP BINDING PROTEIN

OverviewOverview

The 3' end of replication-dependent histone mRNAs terminate in a conserved sequence containing a stem-loop. This 26-nt sequence is the binding site for a protein, stem-loop binding protein (SLBP), that is involved in multiple aspects of histone mRNA metabolism and regulation. We have determined the structure of the 26-nt sequence by multidimensional NMR spectroscopy. There is a 16-nt stem-loop motif, with a conserved 6-bp stem and a 4-nt loop. The loop is closed by a conserved U.A base pair that terminates the canonical A-form stem. The pyrimidine-rich 4-nt loop, UUUC, is well organized with the three uridines stacking on the helix, and the fourth base extending across the major groove into the solvent. The flanking nucleotides at the base of the hairpin stem do not assume a unique conformation, despite the fact that the 5' flanking nucleotides are a critical component of the SLBP binding site.

About this StructureAbout this Structure

1JWC is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

ReferenceReference

NMR structure and dynamics of the RNA-binding site for the histone mRNA stem-loop binding protein., DeJong ES, Marzluff WF, Nikonowicz EP, RNA. 2002 Jan;8(1):83-96. PMID:11871662

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OCA

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-[[Image:1jwc.gif|left|200px]]<br /><applet load="1jwc" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1jwc" />
 '''NMR SOLUTION STRUCTURE OF THE RNA HAIRPIN BINDING SITE FOR THE HISTONE STEM-LOOP BINDING PROTEIN'''<br />
 ==Overview==
-The 3' end of replication-dependent histone mRNAs terminate in a conserved, sequence containing a stem-loop. This 26-nt sequence is the binding site, for a protein, stem-loop binding protein (SLBP), that is involved in, multiple aspects of histone mRNA metabolism and regulation. We have, determined the structure of the 26-nt sequence by multidimensional NMR, spectroscopy. There is a 16-nt stem-loop motif, with a conserved 6-bp stem, and a 4-nt loop. The loop is closed by a conserved U.A base pair that, terminates the canonical A-form stem. The pyrimidine-rich 4-nt loop, UUUC, is well organized with the three uridines stacking on the helix, and the, fourth base extending across the major groove into the solvent. The, flanking nucleotides at the base of the hairpin stem do not assume a, unique conformation, despite the fact that the 5' flanking nucleotides are, a critical component of the SLBP binding site.
+The 3' end of replication-dependent histone mRNAs terminate in a conserved sequence containing a stem-loop. This 26-nt sequence is the binding site for a protein, stem-loop binding protein (SLBP), that is involved in multiple aspects of histone mRNA metabolism and regulation. We have determined the structure of the 26-nt sequence by multidimensional NMR spectroscopy. There is a 16-nt stem-loop motif, with a conserved 6-bp stem and a 4-nt loop. The loop is closed by a conserved U.A base pair that terminates the canonical A-form stem. The pyrimidine-rich 4-nt loop, UUUC, is well organized with the three uridines stacking on the helix, and the fourth base extending across the major groove into the solvent. The flanking nucleotides at the base of the hairpin stem do not assume a unique conformation, despite the fact that the 5' flanking nucleotides are a critical component of the SLBP binding site.
 ==About this Structure==
-JWC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JWC OCA].
+JWC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWC OCA].
 ==Reference==
 NMR structure and dynamics of the RNA-binding site for the histone mRNA stem-loop binding protein., DeJong ES, Marzluff WF, Nikonowicz EP, RNA. 2002 Jan;8(1):83-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11871662 11871662]
 [[Category: Single protein]]
-[[Category: Dejong, E.S.]]
+[[Category: Dejong, E S.]]
-[[Category: Marzluff, W.F.]]
+[[Category: Marzluff, W F.]]
-[[Category: Nikonowicz, E.P.]]
+[[Category: Nikonowicz, E P.]]
 [[Category: 3' stack]]
 [[Category: hairpin]]
 [[Category: tetraloop]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:20:54 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:30 2008''