1jql: Difference between revisions

Revision as of 14:25, 21 February 2008

Mechanism of Processivity Clamp Opening by the Delta Subunit Wrench of the Clamp Loader Complex of E. coli DNA Polymerase III: Structure of beta-delta (1-140)

OverviewOverview

The dimeric ring-shaped sliding clamp of E. coli DNA polymerase III (beta subunit, homolog of eukaryotic PCNA) is loaded onto DNA by the clamp loader gamma complex (homolog of eukaryotic Replication Factor C, RFC). The delta subunit of the gamma complex binds to the beta ring and opens it. The crystal structure of a beta:delta complex shows that delta, which is structurally related to the delta' and gamma subunits of the gamma complex, is a molecular wrench that induces or traps a conformational change in beta such that one of its dimer interfaces is destabilized. Structural comparisons and molecular dynamics simulations suggest a spring-loaded mechanism in which the beta ring opens spontaneously once a dimer interface is perturbed by the delta wrench.

About this StructureAbout this Structure

1JQL is a Protein complex structure of sequences from Escherichia coli. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.

ReferenceReference

Mechanism of processivity clamp opening by the delta subunit wrench of the clamp loader complex of E. coli DNA polymerase III., Jeruzalmi D, Yurieva O, Zhao Y, Young M, Stewart J, Hingorani M, O'Donnell M, Kuriyan J, Cell. 2001 Aug 24;106(4):417-28. PMID:11525728

Page seeded by OCA on Thu Feb 21 13:25:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1jql.jpg|left|200px]]<br /><applet load="1jql" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jql.jpg|left|200px]]<br /><applet load="1jql" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1jql, resolution 2.5&Aring;" />
 '''Mechanism of Processivity Clamp Opening by the Delta Subunit Wrench of the Clamp Loader Complex of E. coli DNA Polymerase III: Structure of beta-delta (1-140)'''<br />
 ==Overview==
-The dimeric ring-shaped sliding clamp of E. coli DNA polymerase III (beta, subunit, homolog of eukaryotic PCNA) is loaded onto DNA by the clamp, loader gamma complex (homolog of eukaryotic Replication Factor C, RFC)., The delta subunit of the gamma complex binds to the beta ring and opens, it. The crystal structure of a beta:delta complex shows that delta, which, is structurally related to the delta' and gamma subunits of the gamma, complex, is a molecular wrench that induces or traps a conformational, change in beta such that one of its dimer interfaces is destabilized., Structural comparisons and molecular dynamics simulations suggest a, spring-loaded mechanism in which the beta ring opens spontaneously once a, dimer interface is perturbed by the delta wrench.
+The dimeric ring-shaped sliding clamp of E. coli DNA polymerase III (beta subunit, homolog of eukaryotic PCNA) is loaded onto DNA by the clamp loader gamma complex (homolog of eukaryotic Replication Factor C, RFC). The delta subunit of the gamma complex binds to the beta ring and opens it. The crystal structure of a beta:delta complex shows that delta, which is structurally related to the delta' and gamma subunits of the gamma complex, is a molecular wrench that induces or traps a conformational change in beta such that one of its dimer interfaces is destabilized. Structural comparisons and molecular dynamics simulations suggest a spring-loaded mechanism in which the beta ring opens spontaneously once a dimer interface is perturbed by the delta wrench.
 ==About this Structure==
-JQL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JQL OCA].
+JQL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQL OCA].
 ==Reference==
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 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
-[[Category: Donnell, M.O.]]
+[[Category: Donnell, M O.]]
 [[Category: Hingorani, M.]]
 [[Category: Jeruzalmi, D.]]
@@ Line 28: / Line 28: @@
 [[Category: processivity clamp]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:29:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:36 2008''