1jox: Difference between revisions

Revision as of 14:25, 21 February 2008

NMR Structure of the LP5.1 Hairpin from Bacillus RNase P RNA Refined with Residual Dipolar Couplings

OverviewOverview

Long-range interactions involving the P5.1 hairpin of Bacillus RNase P RNA are thought to form a structural truss to support RNA folding and activity. We determined the structure of this element by NMR and refined the structure using residual dipolar couplings from a sample weakly oriented in a dilute liquid crystalline mixture of polyethylene glycol and hexanol. Dipolar coupling refinement improved the global precision of the structure from 1.5 to 1.2 A (to the mean), revised the bend angle between segments of the P5.1 stem and corroborated the structure of the loop region. The UGAGAU hexaloop of P5.1 contains two stacks of bases on opposite sides of the loop, distinguishing it from GNRA tetraloops. The unusual conformation of the juxtaposed uracil residues within the hexaloop may explain their requirement in transactivation assays.

About this StructureAbout this Structure

1JOX is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the UGAGAU hexaloop that braces Bacillus RNase P for action., Leeper TC, Martin MB, Kim H, Cox S, Semenchenko V, Schmidt FJ, Van Doren SR, Nat Struct Biol. 2002 May;9(5):397-403. PMID:11927952

Page seeded by OCA on Thu Feb 21 13:25:04 2008

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OCA

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-[[Image:1jox.gif|left|200px]]<br /><applet load="1jox" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1jox" />
 '''NMR Structure of the LP5.1 Hairpin from Bacillus RNase P RNA Refined with Residual Dipolar Couplings'''<br />
 ==Overview==
-Long-range interactions involving the P5.1 hairpin of Bacillus RNase P RNA, are thought to form a structural truss to support RNA folding and, activity. We determined the structure of this element by NMR and refined, the structure using residual dipolar couplings from a sample weakly, oriented in a dilute liquid crystalline mixture of polyethylene glycol and, hexanol. Dipolar coupling refinement improved the global precision of the, structure from 1.5 to 1.2 A (to the mean), revised the bend angle between, segments of the P5.1 stem and corroborated the structure of the loop, region. The UGAGAU hexaloop of P5.1 contains two stacks of bases on, opposite sides of the loop, distinguishing it from GNRA tetraloops. The, unusual conformation of the juxtaposed uracil residues within the hexaloop, may explain their requirement in transactivation assays.
+Long-range interactions involving the P5.1 hairpin of Bacillus RNase P RNA are thought to form a structural truss to support RNA folding and activity. We determined the structure of this element by NMR and refined the structure using residual dipolar couplings from a sample weakly oriented in a dilute liquid crystalline mixture of polyethylene glycol and hexanol. Dipolar coupling refinement improved the global precision of the structure from 1.5 to 1.2 A (to the mean), revised the bend angle between segments of the P5.1 stem and corroborated the structure of the loop region. The UGAGAU hexaloop of P5.1 contains two stacks of bases on opposite sides of the loop, distinguishing it from GNRA tetraloops. The unusual conformation of the juxtaposed uracil residues within the hexaloop may explain their requirement in transactivation assays.
 ==About this Structure==
-JOX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JOX OCA].
+JOX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOX OCA].
 ==Reference==
 Structure of the UGAGAU hexaloop that braces Bacillus RNase P for action., Leeper TC, Martin MB, Kim H, Cox S, Semenchenko V, Schmidt FJ, Van Doren SR, Nat Struct Biol. 2002 May;9(5):397-403. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11927952 11927952]
 [[Category: Protein complex]]
-[[Category: Doren, S.R.Van.]]
+[[Category: Doren, S R.Van.]]
-[[Category: Leeper, T.C.]]
+[[Category: Leeper, T C.]]
-[[Category: Schmidt, F.J.]]
+[[Category: Schmidt, F J.]]
 [[Category: hairpin]]
 [[Category: residual dipolar coupling refinement]]
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 [[Category: ugnrau]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:00:16 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:04 2008''