1jnd: Difference between revisions
New page: left|200px<br /><applet load="1jnd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jnd, resolution 1.3Å" /> '''Crystal structure of ... |
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[[Image:1jnd.gif|left|200px]]<br /><applet load="1jnd" size=" | [[Image:1jnd.gif|left|200px]]<br /><applet load="1jnd" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1jnd, resolution 1.3Å" /> | caption="1jnd, resolution 1.3Å" /> | ||
'''Crystal structure of imaginal disc growth factor-2'''<br /> | '''Crystal structure of imaginal disc growth factor-2'''<br /> | ||
==Overview== | ==Overview== | ||
Imaginal disc growth factor-2 (IDGF-2) is a member of a recently described | Imaginal disc growth factor-2 (IDGF-2) is a member of a recently described family of Drosophila melanogaster-soluble polypeptide growth factors that promote cell proliferation in imaginal discs. Although their precise mode of action has not been established, IDGFs cooperate with insulin in stimulating the growth of imaginal disc cells. We report the crystal structure of IDGF-2 at 1.3-A resolution. The structure shows the classical (betaalpha)(8) barrel-fold of family 18 glycosyl hydrolases, with an insertion of an alpha + beta domain similar to that of Serratia marcescens chitinases A and B. However, amino acid substitutions in the consensus catalytic sequence of chitinases give IDGF-2 a less negatively charged environment in its putative ligand-binding site and preclude the nucleophilic attack mechanism of chitin hydrolysis. Particularly important is the replacement of Glu by Gln at position 132, which has been shown to abolish enzymatic activity in chitinases. Nevertheless, a modest conservation of residues that participate in oligosaccharide recognition suggests that IDGF-2 could bind carbohydrates, assuming several conformational changes to open the partially occluded binding site. Thus, IDGFs may have evolved from chitinases to acquire new functions as growth factors, interacting with cell surface glycoproteins implicated in growth-promoting processes, such as the Drosophila insulin receptor. | ||
==About this Structure== | ==About this Structure== | ||
1JND is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with MAN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1JND is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=MAN:'>MAN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JND OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Llera, A | [[Category: Llera, A S.]] | ||
[[Category: Mariuzza, R | [[Category: Mariuzza, R A.]] | ||
[[Category: Tormo, J.]] | [[Category: Tormo, J.]] | ||
[[Category: Varela, P | [[Category: Varela, P F.]] | ||
[[Category: MAN]] | [[Category: MAN]] | ||
[[Category: chitinase]] | [[Category: chitinase]] | ||
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[[Category: insulin receptor]] | [[Category: insulin receptor]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:32 2008'' | ||
Revision as of 14:24, 21 February 2008
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Crystal structure of imaginal disc growth factor-2
OverviewOverview
Imaginal disc growth factor-2 (IDGF-2) is a member of a recently described family of Drosophila melanogaster-soluble polypeptide growth factors that promote cell proliferation in imaginal discs. Although their precise mode of action has not been established, IDGFs cooperate with insulin in stimulating the growth of imaginal disc cells. We report the crystal structure of IDGF-2 at 1.3-A resolution. The structure shows the classical (betaalpha)(8) barrel-fold of family 18 glycosyl hydrolases, with an insertion of an alpha + beta domain similar to that of Serratia marcescens chitinases A and B. However, amino acid substitutions in the consensus catalytic sequence of chitinases give IDGF-2 a less negatively charged environment in its putative ligand-binding site and preclude the nucleophilic attack mechanism of chitin hydrolysis. Particularly important is the replacement of Glu by Gln at position 132, which has been shown to abolish enzymatic activity in chitinases. Nevertheless, a modest conservation of residues that participate in oligosaccharide recognition suggests that IDGF-2 could bind carbohydrates, assuming several conformational changes to open the partially occluded binding site. Thus, IDGFs may have evolved from chitinases to acquire new functions as growth factors, interacting with cell surface glycoproteins implicated in growth-promoting processes, such as the Drosophila insulin receptor.
About this StructureAbout this Structure
1JND is a Single protein structure of sequence from Drosophila melanogaster with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of imaginal disc growth factor-2. A member of a new family of growth-promoting glycoproteins from Drosophila melanogaster., Varela PF, Llera AS, Mariuzza RA, Tormo J, J Biol Chem. 2002 Apr 12;277(15):13229-36. Epub 2002 Jan 30. PMID:11821393
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