1jjj: Difference between revisions

Revision as of 14:23, 21 February 2008

SOLUTION STRUCTURE OF RECOMBINANT HUMAN EPIDERMAL-TYPE FATTY ACID BINDING PROTEIN

OverviewOverview

Human epidermal-type fatty acid-binding protein (E-FABP) belongs to a family of intracellular 14-15 kDa lipid-binding proteins, whose functions have been associated with fatty acid signalling, cell growth, regulation and differentiation. As a contribution to understanding the structure-function relationship, we report in the present study features of its solution structure and backbone dynamics determined by NMR spectroscopy. Applying multi-dimensional high-resolution NMR techniques on unlabelled and 15N-enriched recombinant human E-FABP, the 1H and 15N resonance assignments were completed. On the basis of 2008 distance restraints, the three-dimensional solution structure of human E-FABP was subsequently obtained (backbone atom root-mean-square deviation of 0.92+/-0.11 A; where 1 A=0.1 nm), consisting mainly of 10 anti-parallel beta-strands that form a beta-barrel structure. 15N relaxation experiments (T1, T2 and heteronuclear nuclear Overhauser effects) at 500, 600 and 800 MHz provided information on the internal dynamics of the protein backbone. Nearly all non-terminal backbone amide groups showed order parameters S(2)>0.8, with an average value of 0.88+/-0.04, suggesting a uniformly low backbone mobility in the nanosecond-to-picosecond time range. Moreover, hydrogen/deuterium exchange experiments indicated a direct correlation between the stability of the hydrogen-bonding network in the beta-sheet structure and the conformational exchange in the millisecond-to-microsecond time range. The features of E-FABP backbone dynamics elaborated in the present study differ markedly from those of the phylogenetically closely related heart-type FABP and the more distantly related ileal lipid-binding protein, implying a strong interdependence with the overall protein stability and possibly also with the ligand-binding affinity for members of the lipid-binding protein family.

About this StructureAbout this Structure

1JJJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Solution structure and backbone dynamics of human epidermal-type fatty acid-binding protein (E-FABP)., Gutierrez-Gonzalez LH, Ludwig C, Hohoff C, Rademacher M, Hanhoff T, Ruterjans H, Spener F, Lucke C, Biochem J. 2002 Jun 15;364(Pt 3):725-37. PMID:12049637

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1jjj.gif|left|200px]]<br />
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 '''SOLUTION STRUCTURE OF RECOMBINANT HUMAN EPIDERMAL-TYPE FATTY ACID BINDING PROTEIN'''<br />
 ==Overview==
-Human epidermal-type fatty acid-binding protein (E-FABP) belongs to a, family of intracellular 14-15 kDa lipid-binding proteins, whose functions, have been associated with fatty acid signalling, cell growth, regulation, and differentiation. As a contribution to understanding the, structure-function relationship, we report in the present study features, of its solution structure and backbone dynamics determined by NMR, spectroscopy. Applying multi-dimensional high-resolution NMR techniques on, unlabelled and 15N-enriched recombinant human E-FABP, the 1H and 15N, resonance assignments were completed. On the basis of 2008 distance, restraints, the three-dimensional solution structure of human E-FABP was, subsequently obtained (backbone atom root-mean-square deviation of, 0.92+/-0.11 A; where 1 A=0.1 nm), consisting mainly of 10 anti-parallel, beta-strands that form a beta-barrel structure. 15N relaxation experiments, (T1, T2 and heteronuclear nuclear Overhauser effects) at 500, 600 and 800, MHz provided information on the internal dynamics of the protein backbone., Nearly all non-terminal backbone amide groups showed order parameters, S(2)&gt;0.8, with an average value of 0.88+/-0.04, suggesting a uniformly low, backbone mobility in the nanosecond-to-picosecond time range. Moreover, hydrogen/deuterium exchange experiments indicated a direct correlation, between the stability of the hydrogen-bonding network in the beta-sheet, structure and the conformational exchange in the, millisecond-to-microsecond time range. The features of E-FABP backbone, dynamics elaborated in the present study differ markedly from those of the, phylogenetically closely related heart-type FABP and the more distantly, related ileal lipid-binding protein, implying a strong interdependence, with the overall protein stability and possibly also with the, ligand-binding affinity for members of the lipid-binding protein family.
+Human epidermal-type fatty acid-binding protein (E-FABP) belongs to a family of intracellular 14-15 kDa lipid-binding proteins, whose functions have been associated with fatty acid signalling, cell growth, regulation and differentiation. As a contribution to understanding the structure-function relationship, we report in the present study features of its solution structure and backbone dynamics determined by NMR spectroscopy. Applying multi-dimensional high-resolution NMR techniques on unlabelled and 15N-enriched recombinant human E-FABP, the 1H and 15N resonance assignments were completed. On the basis of 2008 distance restraints, the three-dimensional solution structure of human E-FABP was subsequently obtained (backbone atom root-mean-square deviation of 0.92+/-0.11 A; where 1 A=0.1 nm), consisting mainly of 10 anti-parallel beta-strands that form a beta-barrel structure. 15N relaxation experiments (T1, T2 and heteronuclear nuclear Overhauser effects) at 500, 600 and 800 MHz provided information on the internal dynamics of the protein backbone. Nearly all non-terminal backbone amide groups showed order parameters S(2)&gt;0.8, with an average value of 0.88+/-0.04, suggesting a uniformly low backbone mobility in the nanosecond-to-picosecond time range. Moreover, hydrogen/deuterium exchange experiments indicated a direct correlation between the stability of the hydrogen-bonding network in the beta-sheet structure and the conformational exchange in the millisecond-to-microsecond time range. The features of E-FABP backbone dynamics elaborated in the present study differ markedly from those of the phylogenetically closely related heart-type FABP and the more distantly related ileal lipid-binding protein, implying a strong interdependence with the overall protein stability and possibly also with the ligand-binding affinity for members of the lipid-binding protein family.
 ==About this Structure==
-JJJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JJJ OCA].
+JJJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJJ OCA].
 ==Reference==
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 [[Category: Homo sapiens]]
 [[Category: Single protein]]
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+[[Category: Gutierrez-Gonzalez, L H.]]
 [[Category: Hanhoff, T.]]
 [[Category: Hohoff, C.]]
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 [[Category: nmr spectroscopy]]
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