1jbr: Difference between revisions
New page: left|200px<br /><applet load="1jbr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jbr, resolution 2.15Å" /> '''Crystal Structure of... |
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[[Image:1jbr.gif|left|200px]]<br /><applet load="1jbr" size=" | [[Image:1jbr.gif|left|200px]]<br /><applet load="1jbr" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1jbr, resolution 2.15Å" /> | caption="1jbr, resolution 2.15Å" /> | ||
'''Crystal Structure of the Ribotoxin Restrictocin and a 31-mer SRD RNA Inhibitor'''<br /> | '''Crystal Structure of the Ribotoxin Restrictocin and a 31-mer SRD RNA Inhibitor'''<br /> | ||
==Overview== | ==Overview== | ||
The cytotoxin sarcin disrupts elongation factor binding and protein | The cytotoxin sarcin disrupts elongation factor binding and protein synthesis by specifically cleaving one phosphodiester bond in ribosomes. To elucidate the molecular basis of toxin action, we determined three cocrystal structures of the sarcin homolog restrictocin bound to different analogs that mimic the target sarcin/ricin loop (SRL) structure of the rat 28S rRNA. In these structures, restrictocin contacts the bulged-G motif and an unfolded form of the tetraloop of the SRL RNA. In one structure, toxin loops guide selection of the target site by contacting the base critical for recognition (G4319) and the surrounding S-shaped backbone. In another structure, base flipping of the tetraloop enables cleavage by placing the target nucleotide in the active site with the nucleophile nearly inline for attack on the scissile bond. These structures provide the first views of how a site-specific protein endonuclease recognizes and cleaves a folded RNA substrate. | ||
==About this Structure== | ==About this Structure== | ||
1JBR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_restrictus Aspergillus restrictus] with K as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1JBR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_restrictus Aspergillus restrictus] with <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBR OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Aspergillus restrictus]] | [[Category: Aspergillus restrictus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Correll, C | [[Category: Correll, C C.]] | ||
[[Category: Gerczei, T.]] | [[Category: Gerczei, T.]] | ||
[[Category: Glover, L.]] | [[Category: Glover, L.]] | ||
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[[Category: specific recognition]] | [[Category: specific recognition]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:20:51 2008'' | ||
Revision as of 14:20, 21 February 2008
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Crystal Structure of the Ribotoxin Restrictocin and a 31-mer SRD RNA Inhibitor
OverviewOverview
The cytotoxin sarcin disrupts elongation factor binding and protein synthesis by specifically cleaving one phosphodiester bond in ribosomes. To elucidate the molecular basis of toxin action, we determined three cocrystal structures of the sarcin homolog restrictocin bound to different analogs that mimic the target sarcin/ricin loop (SRL) structure of the rat 28S rRNA. In these structures, restrictocin contacts the bulged-G motif and an unfolded form of the tetraloop of the SRL RNA. In one structure, toxin loops guide selection of the target site by contacting the base critical for recognition (G4319) and the surrounding S-shaped backbone. In another structure, base flipping of the tetraloop enables cleavage by placing the target nucleotide in the active site with the nucleophile nearly inline for attack on the scissile bond. These structures provide the first views of how a site-specific protein endonuclease recognizes and cleaves a folded RNA substrate.
About this StructureAbout this Structure
1JBR is a Single protein structure of sequence from Aspergillus restrictus with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of restrictocin-inhibitor complexes with implications for RNA recognition and base flipping., Yang X, Gerczei T, Glover LT, Correll CC, Nat Struct Biol. 2001 Nov;8(11):968-73. PMID:11685244
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