1j9k: Difference between revisions

Revision as of 14:20, 21 February 2008

CRYSTAL STRUCTURE OF SURE PROTEIN FROM T.MARITIMA IN COMPLEX WITH TUNGSTATE

OverviewOverview

Homologs of the Escherichia coli surE gene are present in many eubacteria and archaea. Despite the evolutionary conservation, little information is available on the structure and function of their gene products. We have determined the crystal structure of the SurE protein from Thermotoga maritima. The structure reveals the dimeric arrangement of the subunits and an active site around a bound metal ion. We also demonstrate that the SurE protein exhibits a divalent metal ion-dependent phosphatase activity that is inhibited by vanadate or tungstate. In the vanadate- and tungstate-complexed structures, the inhibitors bind adjacent to the divalent metal ion. Our structural and functional analyses identify the SurE proteins as a novel family of metal ion-dependent phosphatases.

About this StructureAbout this Structure

1J9K is a Single protein structure of sequence from Thermotoga maritima with , and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure and functional analysis of the SurE protein identify a novel phosphatase family., Lee JY, Kwak JE, Moon J, Eom SH, Liong EC, Pedelacq JD, Berendzen J, Suh SW, Nat Struct Biol. 2001 Sep;8(9):789-94. PMID:11524683

Page seeded by OCA on Thu Feb 21 13:20:09 2008

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OCA

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-[[Image:1j9k.jpg|left|200px]]<br /><applet load="1j9k" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1j9k.jpg|left|200px]]<br /><applet load="1j9k" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1j9k, resolution 2.10&Aring;" />
 '''CRYSTAL STRUCTURE OF SURE PROTEIN FROM T.MARITIMA IN COMPLEX WITH TUNGSTATE'''<br />
 ==Overview==
-Homologs of the Escherichia coli surE gene are present in many eubacteria, and archaea. Despite the evolutionary conservation, little information is, available on the structure and function of their gene products. We have, determined the crystal structure of the SurE protein from Thermotoga, maritima. The structure reveals the dimeric arrangement of the subunits, and an active site around a bound metal ion. We also demonstrate that the, SurE protein exhibits a divalent metal ion-dependent phosphatase activity, that is inhibited by vanadate or tungstate. In the vanadate- and, tungstate-complexed structures, the inhibitors bind adjacent to the, divalent metal ion. Our structural and functional analyses identify the, SurE proteins as a novel family of metal ion-dependent phosphatases.
+Homologs of the Escherichia coli surE gene are present in many eubacteria and archaea. Despite the evolutionary conservation, little information is available on the structure and function of their gene products. We have determined the crystal structure of the SurE protein from Thermotoga maritima. The structure reveals the dimeric arrangement of the subunits and an active site around a bound metal ion. We also demonstrate that the SurE protein exhibits a divalent metal ion-dependent phosphatase activity that is inhibited by vanadate or tungstate. In the vanadate- and tungstate-complexed structures, the inhibitors bind adjacent to the divalent metal ion. Our structural and functional analyses identify the SurE proteins as a novel family of metal ion-dependent phosphatases.
 ==About this Structure==
-J9K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with CA, WO4 and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J9K OCA].
+J9K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=WO4:'>WO4</scene> and <scene name='pdbligand=EPE:'>EPE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J9K OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Thermotoga maritima]]
-[[Category: Kwak, J.E.]]
+[[Category: Kwak, J E.]]
-[[Category: Lee, J.Y.]]
+[[Category: Lee, J Y.]]
 [[Category: Moon, J.]]
-[[Category: Suh, S.W.]]
+[[Category: Suh, S W.]]
 [[Category: CA]]
 [[Category: EPE]]
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 [[Category: tungstate complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:01:54 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:20:09 2008''