StudioG24SecL04Tpc5: Difference between revisions
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Although VlsE is able to evade immune response and maintain persistent infection through antigenic variation, a peptide associated with IR6 has been found to have seriodiagnostic properties in Lyme disease<ref>http://blackboard.stonybrook.edu/bbcswebdav/pid-2393240-dt-content-rid-7501335_1/courses/1126-BIO-207-SEC30-66348/Eicken%20et%20al%202002%281%29.pdf/</ref>. | Although VlsE is able to evade immune response and maintain persistent infection through antigenic variation, a peptide associated with IR6 has been found to have seriodiagnostic properties in Lyme disease<ref>http://blackboard.stonybrook.edu/bbcswebdav/pid-2393240-dt-content-rid-7501335_1/courses/1126-BIO-207-SEC30-66348/Eicken%20et%20al%202002%281%29.pdf/</ref>. | ||
In a comparative study of human sera using recombinant antigens of ''B. Burgdorferi'', the samples displayed 80% seropositivity when studied for IgG antibodies when examined by ELISAs<ref>http://jmm.sgmjournals.org/content/51/8/649.full.pdf/</ref> . It suggests that the invariable regions of VlsE may serve as useful universal probes for Lyme disease. The sera sampled by the study were from the archives in Connecticut of patients who resided in the United States<ref>http://jmm.sgmjournals.org/content/51/8/649.full.pdf/</ref>. However, Lyme disease is not exclusive to the United States therefore further analysis of VlsE reactivity with Lyme disease borreliosis from broad geographical regions is required<ref>http://jmm.sgmjournals.org/content/51/8/649.full.pdf/</ref>. | In a comparative study of human sera using recombinant antigens of ''B. Burgdorferi'', the samples displayed 80% seropositivity when studied for IgG antibodies when examined by ELISAs<ref>http://jmm.sgmjournals.org/content/51/8/649.full.pdf/</ref> . It suggests that the invariable regions of VlsE may serve as useful universal probes for Lyme disease. The sera sampled by the study were from the archives in Connecticut of patients who resided in the United States<ref>http://jmm.sgmjournals.org/content/51/8/649.full.pdf/</ref>. However, Lyme disease is not exclusive to the United States therefore further analysis of VlsE reactivity with Lyme disease borreliosis from broad geographical regions is required<ref>http://jmm.sgmjournals.org/content/51/8/649.full.pdf/</ref>. | ||
==Future Research== | |||
''vlsE'' recombination is rapid during the infection process in immunocompetent or immunodeficient mice. However, it has not been detected during ''in vitro'' culture. Ohnishi J, P. J. and de Silva A. M. reported that ticks inoculated via feeding on infected mice containing ''vlsE'' sequence variants that either arose within the ticks or selected during the second feeding.<ref name="A">PMID:10569796</ref> However, vlsE recombination is not detected by recent studies conducted by Indest, K.J. that inoculated ticks with ''B. burgdorferi'' clone 5A3 via capillary feeding.<ref name="A">PMID:10569796</ref>Present research explored the recombination mechanism and whether it is up-regulated in mammalian tissues in comparison with either the arthropod of ''in vitro'' culture environments. | |||