G09SecL04Tpc2: Difference between revisions
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Outer Surface Protein A has approximately 270 amino acid residues. <ref name=art3>PMID:11183781</ref>In terms of secondary structure, OspA is made up of 21 anti-parallel single layer beta strands and one alpha helix. <ref name=art3>PMID:11183781</ref>OspA has three major components to its structure: an <scene name='G09SecL04Tpc2/3sites/3'>n-terminal sandwich</scene>, <scene name='G09SecL04Tpc2/3sites/2'>a central sheet</scene>, and a <scene name='G09SecL04Tpc2/3sites/4'>c-terminal barrel domain</scene>. The n-terminal is often termed as a sandwich due to its jumbled formation of amino acid residues. Most of the beta strands lie on the central sheet. Lastly, the c-terminal is called barrel domains because the three primary loops connect to both sides of the central beta sheet forming a barrel or a hole in the middle. This can be seen by rotating the molecule. | Outer Surface Protein A has approximately 270 amino acid residues. <ref name=art3>PMID:11183781</ref>In terms of secondary structure, OspA is made up of 21 anti-parallel single layer beta strands and one alpha helix. <ref name=art3>PMID:11183781</ref>OspA has three major components to its structure: an <scene name='G09SecL04Tpc2/3sites/3'>n-terminal sandwich</scene>, <scene name='G09SecL04Tpc2/3sites/2'>a central sheet</scene>, and a <scene name='G09SecL04Tpc2/3sites/4'>c-terminal barrel domain</scene>. The n-terminal is often termed as a sandwich due to its jumbled formation of amino acid residues. Most of the beta strands lie on the central sheet. Lastly, the c-terminal is called barrel domains because the three primary loops connect to both sides of the central beta sheet forming a barrel or a hole in the middle. This can be seen by rotating the molecule. | ||
The c-terminal is heavily involved in the antigen:antibody complex unlike the n-terminal. The c-terminal houses a protruding ridge of three loops: <scene name='G09SecL04Tpc2/3loops/1'>loop 1</scene> contains residues 203-220; <scene name='G09SecL04Tpc2/3loops/2'>loop 2</scene> contains residues 224-233; <scene name='G09SecL04Tpc2/3loops/3'>loop 3</scene> contains residues 246-257. These loops define the LA-2 (antibody) epitope. More specifically, the loops indicate where the antibody binds to the antigen. This protruding ridge coupled with OspA’s elongated fold gives the surface protein a high degree of mobility and surface exposure. | The c-terminal is heavily involved in the antigen:antibody complex unlike the n-terminal. The c-terminal houses a protruding ridge of three loops: <scene name='G09SecL04Tpc2/3loops/1'>loop 1</scene> contains residues 203-220; <scene name='G09SecL04Tpc2/3loops/2'>loop 2</scene> contains residues 224-233; <scene name='G09SecL04Tpc2/3loops/3'>loop 3</scene> contains residues 246-257. These loops define the LA-2 (antibody) [http://en.wikipedia.org/wiki/Epitope epitope]. More specifically, the loops indicate where the antibody binds to the antigen. This protruding ridge coupled with OspA’s elongated fold gives the surface protein a high degree of mobility and surface exposure. | ||
=== LA-2 Recognition === | === LA-2 Recognition === | ||