G18secL03Tpc4: Difference between revisions
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==== Putative Binding Site ==== | ==== Putative Binding Site ==== | ||
<Structure load='1ggq' size='400' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' /> | <Structure load='1ggq' size='400' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' /> | ||
The binding site of OspC is believed to be located on the surface that projects away from the membrane and has a region with strong negative electrostatic potential. Cavities are formed at the top of the molecule away from the membrane surface. Each cavity has a volume of 50 Å3 and is formed by residues Ala75, Ile76, Gly77, Lys78, Lys79, Glu89, Ala90, Asp91, His92 and Asn93 of one monomer, and Gly94, Ser95, Ser98, Gly146, Lys147 and Glu148 of the other monomer.<ref>D. Kumaran1, S. Eswaramoorthy1, B.J. Luft2, S. Koide3, J.J. Dunn1, C.L. Lawson1,4 and S. Swaminathan1. Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi.The EMBO Journal (2001) 20, 971 - 978 [http://dx.doi.org/DOI:10.1093/emboj/20.5.971]</ref> Positively charged Magnesium ion | The <scene name='G18secL03Tpc4/Binding_site/1'>binding site</scene> of OspC is believed to be located on the surface that projects away from the membrane and has a region with strong negative electrostatic potential. Cavities are formed at the top of the molecule away from the membrane surface. Each cavity has a volume of 50 Å3 and is formed by residues Ala75, Ile76, Gly77, Lys78, Lys79, Glu89, Ala90, Asp91, His92 and Asn93 of one monomer, and Gly94, Ser95, Ser98, Gly146, Lys147 and Glu148 of the other monomer.<ref>D. Kumaran1, S. Eswaramoorthy1, B.J. Luft2, S. Koide3, J.J. Dunn1, C.L. Lawson1,4 and S. Swaminathan1. Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi.The EMBO Journal (2001) 20, 971 - 978 [http://dx.doi.org/DOI:10.1093/emboj/20.5.971]</ref> Positively charged Magnesium ion | ||
<scene name='G18secL03Tpc4/Mg_ion/1'>Mg2+</scene> between the two dimers demonstrates the location of hypothesized binding site. | <scene name='G18secL03Tpc4/Mg_ion/1'>Mg2+</scene> between the two dimers demonstrates the location of hypothesized binding site. | ||
=== Role of OspC in Lyme Disease === | === Role of OspC in Lyme Disease === | ||