1ixi: Difference between revisions
New page: left|200px<br /><applet load="1ixi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ixi, resolution 1.89Å" /> '''PHOSPHATE-BINDING PR... |
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[[Image:1ixi.gif|left|200px]]<br /><applet load="1ixi" size=" | [[Image:1ixi.gif|left|200px]]<br /><applet load="1ixi" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1ixi, resolution 1.89Å" /> | caption="1ixi, resolution 1.89Å" /> | ||
'''PHOSPHATE-BINDING PROTEIN MUTANT WITH ASP 56 REPLACED BY ASN COMPLEX WITH MONOBASIC PHOSPHATE ION'''<br /> | '''PHOSPHATE-BINDING PROTEIN MUTANT WITH ASP 56 REPLACED BY ASN COMPLEX WITH MONOBASIC PHOSPHATE ION'''<br /> | ||
==Overview== | ==Overview== | ||
A very short hydrogen bond between an Asp and a phosphate is established | A very short hydrogen bond between an Asp and a phosphate is established in two high resolution structures (0.98 and 1.05 A). A mutant complex that changes the Asp to an Asn, which forms a normal hydrogen bond, has a similar free energy of binding to the wild type complex, suggesting that the contribution of the short hydrogen bond is not extraordinarily strong. | ||
==About this Structure== | ==About this Structure== | ||
1IXI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with 2HP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1IXI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=2HP:'>2HP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IXI OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Quiocho, F | [[Category: Quiocho, F A.]] | ||
[[Category: Wang, Z.]] | [[Category: Wang, Z.]] | ||
[[Category: 2HP]] | [[Category: 2HP]] | ||
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[[Category: phosphate-binding]] | [[Category: phosphate-binding]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:16:43 2008'' | ||
Revision as of 14:16, 21 February 2008
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PHOSPHATE-BINDING PROTEIN MUTANT WITH ASP 56 REPLACED BY ASN COMPLEX WITH MONOBASIC PHOSPHATE ION
OverviewOverview
A very short hydrogen bond between an Asp and a phosphate is established in two high resolution structures (0.98 and 1.05 A). A mutant complex that changes the Asp to an Asn, which forms a normal hydrogen bond, has a similar free energy of binding to the wild type complex, suggesting that the contribution of the short hydrogen bond is not extraordinarily strong.
About this StructureAbout this Structure
1IXI is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
A low energy short hydrogen bond in very high resolution structures of protein receptor--phosphate complexes., Wang Z, Luecke H, Yao N, Quiocho FA, Nat Struct Biol. 1997 Jul;4(7):519-22. PMID:9228942
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