2c8l: Difference between revisions

Revision as of 15:16, 30 October 2007

CRYSTAL STRUCTURE OF (SR) CALCIUM-ATPASE E2(TG) FORM

OverviewOverview

We present crystal structures of the calcium-free E2 state of the, sarcoplasmic reticulum Ca2+ -ATPase, stabilized by the inhibitor, thapsigargin and the ATP analog AMPPCP. The structures allow us to, describe the ATP binding site in a modulatory mode uncoupled from the, Asp351 phosphorylation site. The Glu439 side chain interacts with AMPPCP, via an Mg2+ ion in accordance with previous Fe2+ -cleavage studies, implicating this residue in the ATPase cycle and in magnesium binding., Functional data on Ca2+ mediated activation indicate that the crystallized, state represents an initial stage of ATP modulated deprotonation of E2, preceding the binding of Ca2+ ions in the membrane from the cytoplasmic, side. We propose a mechanism of Ca2+ activation of phosphorylation leading, directly from ... [(full description)]

About this StructureAbout this Structure

2C8L is a [Single protein] structure of sequence from [Oryctolagus cuniculus] with NA and TG1 as [ligands]. Active as [Calcium-transporting ATPase], with EC number [3.6.3.8]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

ReferenceReference

Modulatory and catalytic modes of ATP binding by the calcium pump., Jensen AM, Sorensen TL, Olesen C, Moller JV, Nissen P, EMBO J. 2006 Jun 7;25(11):2305-14. Epub 2006 May 18. PMID:16710301

Page seeded by OCA on Tue Oct 30 14:21:25 2007

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 ==About this Structure==
-C8L is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]] with NA and TG1 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C8L OCA]].
+C8L is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]] with NA and TG1 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C8L OCA]].
 ==Reference==
 Modulatory and catalytic modes of ATP binding by the calcium pump., Jensen AM, Sorensen TL, Olesen C, Moller JV, Nissen P, EMBO J. 2006 Jun 7;25(11):2305-14. Epub 2006 May 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16710301 16710301]
+[[Category: Calcium-transporting ATPase]]
 [[Category: Oryctolagus cuniculus]]
 [[Category: Single protein]]
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 [[Category: phosphorylation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:54:02 2007''
+''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:21:25 2007''