1ibm: Difference between revisions

Revision as of 14:10, 21 February 2008

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH A MESSENGER RNA FRAGMENT AND COGNATE TRANSFER RNA ANTICODON STEM-LOOP BOUND AT THE A SITE

OverviewOverview

Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and essential bases A1492, A1493, and G530 of 16S RNA. These bases interact intimately with the minor groove of the first two base pairs between the codon and anticodon, thus sensing Watson-Crick base-pairing geometry and discriminating against near-cognate tRNA. The third, or "wobble," position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs.

About this StructureAbout this Structure

1IBM is a Protein complex structure of sequences from Thermus thermophilus with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Recognition of cognate transfer RNA by the 30S ribosomal subunit., Ogle JM, Brodersen DE, Clemons WM Jr, Tarry MJ, Carter AP, Ramakrishnan V, Science. 2001 May 4;292(5518):897-902. PMID:11340196

Page seeded by OCA on Thu Feb 21 13:10:12 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1ibm.jpg|left|200px]]<br /><applet load="1ibm" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ibm.jpg|left|200px]]<br /><applet load="1ibm" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ibm, resolution 3.31&Aring;" />
 '''STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH A MESSENGER RNA FRAGMENT AND COGNATE TRANSFER RNA ANTICODON STEM-LOOP BOUND AT THE A SITE'''<br />
 ==Overview==
-Crystal structures of the 30S ribosomal subunit in complex with messenger, RNA and cognate transfer RNA in the A site, both in the presence and, absence of the antibiotic paromomycin, have been solved at between 3.1 and, 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces, global domain movements of the 30S subunit and changes in the conformation, of the universally conserved and essential bases A1492, A1493, and G530 of, 16S RNA. These bases interact intimately with the minor groove of the, first two base pairs between the codon and anticodon, thus sensing, Watson-Crick base-pairing geometry and discriminating against near-cognate, tRNA. The third, or "wobble," position of the codon is free to accommodate, certain noncanonical base pairs. By partially inducing these structural, changes, paromomycin facilitates binding of near-cognate tRNAs.
+Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and essential bases A1492, A1493, and G530 of 16S RNA. These bases interact intimately with the minor groove of the first two base pairs between the codon and anticodon, thus sensing Watson-Crick base-pairing geometry and discriminating against near-cognate tRNA. The third, or "wobble," position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs.
 ==About this Structure==
-IBM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MG and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IBM OCA].
+IBM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IBM OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Thermus thermophilus]]
-[[Category: Brodersen, D.E.]]
+[[Category: Brodersen, D E.]]
-[[Category: Carter, A.P.]]
+[[Category: Carter, A P.]]
-[[Category: Jr., W.M.Clemons.]]
+[[Category: Jr., W M.Clemons.]]
-[[Category: Ogle, J.M.]]
+[[Category: Ogle, J M.]]
 [[Category: Ramakrishnan, V.]]
-[[Category: Tarry, M.J.]]
+[[Category: Tarry, M J.]]
 [[Category: MG]]
 [[Category: ZN]]
@@ Line 32: / Line 32: @@
 [[Category: transfer trna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:15:34 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:12 2008''