1i8l: Difference between revisions
New page: left|200px<br /> <applet load="1i8l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i8l, resolution 3.00Å" /> '''HUMAN B7-1/CTLA-4 C... |
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[[Image:1i8l.gif|left|200px]]<br /> | [[Image:1i8l.gif|left|200px]]<br /><applet load="1i8l" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1i8l, resolution 3.00Å" /> | caption="1i8l, resolution 3.00Å" /> | ||
'''HUMAN B7-1/CTLA-4 CO-STIMULATORY COMPLEX'''<br /> | '''HUMAN B7-1/CTLA-4 CO-STIMULATORY COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
Optimal immune responses require both an antigen-specific and a | Optimal immune responses require both an antigen-specific and a co-stimulatory signal. The shared ligands B7-1 and B7-2 on antigen-presenting cells deliver the co-stimulatory signal through CD28 and CTLA-4 on T cells. Signalling through CD28 augments the T-cell response, whereas CTLA-4 signalling attenuates it. Numerous animal studies and recent clinical trials indicate that manipulating these interactions holds considerable promise for immunotherapy. With the consequences of these signals well established, and details of the downstream signalling events emerging, understanding the molecular nature of these extracellular interactions becomes crucial. Here we report the crystal structure of the human CTLA-4/B7-1 co-stimulatory complex at 3.0 A resolution. In contrast to other interacting cell-surface molecules, the relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. In the crystal lattice, CTLA-4 and B7-1 pack in a strikingly periodic arrangement in which bivalent CTLA-4 homodimers bridge bivalent B7-1 homodimers. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1I8L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG and MAN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1I8L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=MAN:'>MAN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8L OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Mosyak, L.]] | [[Category: Mosyak, L.]] | ||
[[Category: Somers, W | [[Category: Somers, W S.]] | ||
[[Category: Stamper, C | [[Category: Stamper, C C.]] | ||
[[Category: MAN]] | [[Category: MAN]] | ||
[[Category: NAG]] | [[Category: NAG]] | ||
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[[Category: receptors]] | [[Category: receptors]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:08 2008'' | ||
Revision as of 14:09, 21 February 2008
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HUMAN B7-1/CTLA-4 CO-STIMULATORY COMPLEX
OverviewOverview
Optimal immune responses require both an antigen-specific and a co-stimulatory signal. The shared ligands B7-1 and B7-2 on antigen-presenting cells deliver the co-stimulatory signal through CD28 and CTLA-4 on T cells. Signalling through CD28 augments the T-cell response, whereas CTLA-4 signalling attenuates it. Numerous animal studies and recent clinical trials indicate that manipulating these interactions holds considerable promise for immunotherapy. With the consequences of these signals well established, and details of the downstream signalling events emerging, understanding the molecular nature of these extracellular interactions becomes crucial. Here we report the crystal structure of the human CTLA-4/B7-1 co-stimulatory complex at 3.0 A resolution. In contrast to other interacting cell-surface molecules, the relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. In the crystal lattice, CTLA-4 and B7-1 pack in a strikingly periodic arrangement in which bivalent CTLA-4 homodimers bridge bivalent B7-1 homodimers. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses.
DiseaseDisease
Known diseases associated with this structure: Celiac disease, susceptibility to OMIM:[123890], Diabetes mellitus, insulin-dependent, susceptibility to OMIM:[123890], Graves disease, susceptibility to OMIM:[123890], Hypothyroidism, autoimmune OMIM:[123890]
About this StructureAbout this Structure
1I8L is a Protein complex structure of sequences from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the B7-1/CTLA-4 complex that inhibits human immune responses., Stamper CC, Zhang Y, Tobin JF, Erbe DV, Ikemizu S, Davis SJ, Stahl ML, Seehra J, Somers WS, Mosyak L, Nature. 2001 Mar 29;410(6828):608-11. PMID:11279502
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