1i3q: Difference between revisions

Revision as of 14:07, 21 February 2008

RNA POLYMERASE II CRYSTAL FORM I AT 3.1 A RESOLUTION

OverviewOverview

Structures of a 10-subunit yeast RNA polymerase II have been derived from two crystal forms at 2.8 and 3.1 angstrom resolution. Comparison of the structures reveals a division of the polymerase into four mobile modules, including a clamp, shown previously to swing over the active center. In the 2.8 angstrom structure, the clamp is in an open state, allowing entry of straight promoter DNA for the initiation of transcription. Three loops extending from the clamp may play roles in RNA unwinding and DNA rewinding during transcription. A 2.8 angstrom difference Fourier map reveals two metal ions at the active site, one persistently bound and the other possibly exchangeable during RNA synthesis. The results also provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain.

About this StructureAbout this Structure

1I3Q is a Protein complex structure of sequences from Saccharomyces cerevisiae with and as ligands. This structure supersedes the now removed PDB entry 1EN0. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution., Cramer P, Bushnell DA, Kornberg RD, Science. 2001 Jun 8;292(5523):1863-76. Epub 2001 Apr 19. PMID:11313498

Page seeded by OCA on Thu Feb 21 13:07:38 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1i3q.gif|left|200px]]<br /><applet load="1i3q" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1i3q.gif|left|200px]]<br /><applet load="1i3q" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1i3q, resolution 3.1&Aring;" />
 '''RNA POLYMERASE II CRYSTAL FORM I AT 3.1 A RESOLUTION'''<br />
 ==Overview==
-Structures of a 10-subunit yeast RNA polymerase II have been derived from, two crystal forms at 2.8 and 3.1 angstrom resolution. Comparison of the, structures reveals a division of the polymerase into four mobile modules, including a clamp, shown previously to swing over the active center. In, the 2.8 angstrom structure, the clamp is in an open state, allowing entry, of straight promoter DNA for the initiation of transcription. Three loops, extending from the clamp may play roles in RNA unwinding and DNA rewinding, during transcription. A 2.8 angstrom difference Fourier map reveals two, metal ions at the active site, one persistently bound and the other, possibly exchangeable during RNA synthesis. The results also provide, evidence for RNA exit in the vicinity of the carboxyl-terminal repeat, domain, coupling synthesis to RNA processing by enzymes bound to this, domain.
+Structures of a 10-subunit yeast RNA polymerase II have been derived from two crystal forms at 2.8 and 3.1 angstrom resolution. Comparison of the structures reveals a division of the polymerase into four mobile modules, including a clamp, shown previously to swing over the active center. In the 2.8 angstrom structure, the clamp is in an open state, allowing entry of straight promoter DNA for the initiation of transcription. Three loops extending from the clamp may play roles in RNA unwinding and DNA rewinding during transcription. A 2.8 angstrom difference Fourier map reveals two metal ions at the active site, one persistently bound and the other possibly exchangeable during RNA synthesis. The results also provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain.
 ==About this Structure==
-I3Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 1EN0. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I3Q OCA].
+I3Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1EN0. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3Q OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Protein complex]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Bushnell, D.A.]]
+[[Category: Bushnell, D A.]]
 [[Category: Cramer, P.]]
-[[Category: Kornberg, R.D.]]
+[[Category: Kornberg, R D.]]
 [[Category: MG]]
 [[Category: ZN]]
@@ Line 25: / Line 25: @@
 [[Category: zinc motifs]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:02:03 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:07:38 2008''