1hqz: Difference between revisions

Revision as of 14:04, 21 February 2008

Cofilin homology domain of a yeast actin-binding protein ABP1P

OverviewOverview

A modified molecular-replacement method is described that makes use of six-dimensional searches and the phased translation function, providing a systematic examination of all possible search-model orientations in an experimental electron-density map. As an example, the structure solution of the cofilin-homology domain of the Saccharomyces cerevisiae actin-binding protein 1 (ABP1) is presented in detail. Additional examples are presented in which these tools have significantly aided structure solutions in a variety of contexts. These results suggest that this approach might be of widespread utility for challenging structures involving weak phase information, complex asymmetric units and search models with weak structural homology. Furthermore, this approach supports an exhaustive molecular-replacement strategy in cases where an appropriate search model cannot readily be identified on the basis of sequence homology. The fully automated web-based implementation of this phased translation function is described.

About this StructureAbout this Structure

1HQZ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Phased translation function revisited: structure solution of the cofilin-homology domain from yeast actin-binding protein 1 using six-dimensional searches., Strokopytov BV, Fedorov A, Mahoney NM, Kessels M, Drubin DG, Almo SC, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):285-93. Epub 2005, Feb 24. PMID:15735338

Page seeded by OCA on Thu Feb 21 13:04:11 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1hqz.gif|left|200px]]<br /><applet load="1hqz" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1hqz.gif|left|200px]]<br /><applet load="1hqz" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1hqz, resolution 2.10&Aring;" />
 '''Cofilin homology domain of a yeast actin-binding protein ABP1P'''<br />
 ==Overview==
-A modified molecular-replacement method is described that makes use of, six-dimensional searches and the phased translation function, providing a, systematic examination of all possible search-model orientations in an, experimental electron-density map. As an example, the structure solution, of the cofilin-homology domain of the Saccharomyces cerevisiae, actin-binding protein 1 (ABP1) is presented in detail. Additional examples, are presented in which these tools have significantly aided structure, solutions in a variety of contexts. These results suggest that this, approach might be of widespread utility for challenging structures, involving weak phase information, complex asymmetric units and search, models with weak structural homology. Furthermore, this approach supports, an exhaustive molecular-replacement strategy in cases where an appropriate, search model cannot readily be identified on the basis of sequence, homology. The fully automated web-based implementation of this phased, translation function is described.
+A modified molecular-replacement method is described that makes use of six-dimensional searches and the phased translation function, providing a systematic examination of all possible search-model orientations in an experimental electron-density map. As an example, the structure solution of the cofilin-homology domain of the Saccharomyces cerevisiae actin-binding protein 1 (ABP1) is presented in detail. Additional examples are presented in which these tools have significantly aided structure solutions in a variety of contexts. These results suggest that this approach might be of widespread utility for challenging structures involving weak phase information, complex asymmetric units and search models with weak structural homology. Furthermore, this approach supports an exhaustive molecular-replacement strategy in cases where an appropriate search model cannot readily be identified on the basis of sequence homology. The fully automated web-based implementation of this phased translation function is described.
 ==About this Structure==
-HQZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HQZ OCA].
+HQZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQZ OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
-[[Category: Almo, S.C.]]
+[[Category: Almo, S C.]]
-[[Category: Burley, S.K.]]
+[[Category: Burley, S K.]]
-[[Category: Drubin, D.G.]]
+[[Category: Drubin, D G.]]
-[[Category: Fedorov, A.A.]]
+[[Category: Fedorov, A A.]]
 [[Category: Mahoney, N.]]
-[[Category: NYSGXRC, New.York.Structural.GenomiX.Research.Consortium.]]
+[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
-[[Category: Strokopytov, B.V.]]
+[[Category: Strokopytov, B V.]]
 [[Category: actin binding]]
 [[Category: cofilin homology domain]]
@@ Line 28: / Line 28: @@
 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:45:56 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:04:11 2008''