1hqw: Difference between revisions

Revision as of 14:03, 21 February 2008

CRYSTAL STRUCTURE OF THE COMPLEX OF CONCANAVALIN A WITH A TRIPEPTIDE YPY

OverviewOverview

The X-ray structure analysis of a cross-linked crystal of concanavalin A soaked with the tripeptide molecule as the probe molecule showed electron density corresponding to full occupation in the binding pocket. The site lies on the surface of concanavalin A and is surrounded by three symmetry-related molecules. The crystal structure of the tripeptide complex was refined at 2.4-A resolution to an R-factor of 17.5%, (Rfree factor of 23.7%), with an RMS deviation in bond distances of 0.01 A. The model includes all 237 residue of concanavalin A, 1 manganese ion, 1 calcium ion, 161 water molecules, 1 glutaraldehyde molecule, and 1 tripeptide molecule. This X-ray structure analysis also provides an approach to mapping the binding surface of crystalline protein with a probe molecule that is dissolved in a mixture of organic solvent with water or in neat organic solvent but is hardly dissolved in aqueous solution.

About this StructureAbout this Structure

1HQW is a Single protein structure of sequence from Canavalia ensiformis with , and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the complex of concanavalin A and tripeptide., Zhang Z, Qian M, Huang Q, Jia Y, Tang Y, Wang K, Cui D, Li M, J Protein Chem. 2001 Jan;20(1):59-65. PMID:11330349

Page seeded by OCA on Thu Feb 21 13:03:57 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1hqw.gif|left|200px]]<br /><applet load="1hqw" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1hqw.gif|left|200px]]<br /><applet load="1hqw" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1hqw, resolution 2.40&Aring;" />
 '''CRYSTAL STRUCTURE OF THE COMPLEX OF CONCANAVALIN A WITH A TRIPEPTIDE YPY'''<br />
 ==Overview==
-The X-ray structure analysis of a cross-linked crystal of concanavalin A, soaked with the tripeptide molecule as the probe molecule showed electron, density corresponding to full occupation in the binding pocket. The site, lies on the surface of concanavalin A and is surrounded by three, symmetry-related molecules. The crystal structure of the tripeptide, complex was refined at 2.4-A resolution to an R-factor of 17.5%, (Rfree, factor of 23.7%), with an RMS deviation in bond distances of 0.01 A. The, model includes all 237 residue of concanavalin A, 1 manganese ion, 1, calcium ion, 161 water molecules, 1 glutaraldehyde molecule, and 1, tripeptide molecule. This X-ray structure analysis also provides an, approach to mapping the binding surface of crystalline protein with a, probe molecule that is dissolved in a mixture of organic solvent with, water or in neat organic solvent but is hardly dissolved in aqueous, solution.
+The X-ray structure analysis of a cross-linked crystal of concanavalin A soaked with the tripeptide molecule as the probe molecule showed electron density corresponding to full occupation in the binding pocket. The site lies on the surface of concanavalin A and is surrounded by three symmetry-related molecules. The crystal structure of the tripeptide complex was refined at 2.4-A resolution to an R-factor of 17.5%, (Rfree factor of 23.7%), with an RMS deviation in bond distances of 0.01 A. The model includes all 237 residue of concanavalin A, 1 manganese ion, 1 calcium ion, 161 water molecules, 1 glutaraldehyde molecule, and 1 tripeptide molecule. This X-ray structure analysis also provides an approach to mapping the binding surface of crystalline protein with a probe molecule that is dissolved in a mixture of organic solvent with water or in neat organic solvent but is hardly dissolved in aqueous solution.
 ==About this Structure==
-HQW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with MN, CA and PTD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HQW OCA].
+HQW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=PTD:'>PTD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQW OCA].
 ==Reference==
@@ Line 26: / Line 26: @@
 [[Category: beta sheets]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:45:45 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:03:57 2008''