1hh2: Difference between revisions

Revision as of 14:01, 21 February 2008

CRYSTAL STRUCTURE OF NUSA FROM THERMOTOGA MARITIMA

OverviewOverview

The crystal structure of Thermotoga maritima NusA, a transcription factor involved in pausing, termination, and antitermination processes, reveals a four-domain, rod-shaped molecule. An N-terminal alpha/beta portion, a five-stranded beta-barrel (S1 domain), and two K-homology (KH) modules create a continuous spine of positive electrostatic potential, suitable for nonspecific mRNA attraction. Homology models suggest how, in addition, specific mRNA regulatory sequences can be recognized by the S1 and KH motifs. An arrangement of multiple S1 and KH domains mediated by highly conserved residues is seen, creating an extended RNA binding surface, a paradigm for other proteins with similar domain arrays. Structural and mutational analyses indicate that the motifs cooperate, modulating strength and specificity of RNA binding.

About this StructureAbout this Structure

1HH2 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

ReferenceReference

An extended RNA binding surface through arrayed S1 and KH domains in transcription factor NusA., Worbs M, Bourenkov GP, Bartunik HD, Huber R, Wahl MC, Mol Cell. 2001 Jun;7(6):1177-89. PMID:11430821

Page seeded by OCA on Thu Feb 21 13:01:11 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1hh2.jpg|left|200px]]<br /><applet load="1hh2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1hh2.jpg|left|200px]]<br /><applet load="1hh2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1hh2, resolution 2.1&Aring;" />
 '''CRYSTAL STRUCTURE OF NUSA FROM THERMOTOGA MARITIMA'''<br />
 ==Overview==
-The crystal structure of Thermotoga maritima NusA, a transcription factor, involved in pausing, termination, and antitermination processes, reveals a, four-domain, rod-shaped molecule. An N-terminal alpha/beta portion, a, five-stranded beta-barrel (S1 domain), and two K-homology (KH) modules, create a continuous spine of positive electrostatic potential, suitable, for nonspecific mRNA attraction. Homology models suggest how, in addition, specific mRNA regulatory sequences can be recognized by the S1 and KH, motifs. An arrangement of multiple S1 and KH domains mediated by highly, conserved residues is seen, creating an extended RNA binding surface, a, paradigm for other proteins with similar domain arrays. Structural and, mutational analyses indicate that the motifs cooperate, modulating, strength and specificity of RNA binding.
+The crystal structure of Thermotoga maritima NusA, a transcription factor involved in pausing, termination, and antitermination processes, reveals a four-domain, rod-shaped molecule. An N-terminal alpha/beta portion, a five-stranded beta-barrel (S1 domain), and two K-homology (KH) modules create a continuous spine of positive electrostatic potential, suitable for nonspecific mRNA attraction. Homology models suggest how, in addition, specific mRNA regulatory sequences can be recognized by the S1 and KH motifs. An arrangement of multiple S1 and KH domains mediated by highly conserved residues is seen, creating an extended RNA binding surface, a paradigm for other proteins with similar domain arrays. Structural and mutational analyses indicate that the motifs cooperate, modulating strength and specificity of RNA binding.
 ==About this Structure==
-HH2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HH2 OCA].
+HH2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH2 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Thermotoga maritima]]
-[[Category: Bartunik, H.D.]]
+[[Category: Bartunik, H D.]]
-[[Category: Bourenkov, G.P.]]
+[[Category: Bourenkov, G P.]]
 [[Category: Huber, R.]]
-[[Category: Wahl, M.C.]]
+[[Category: Wahl, M C.]]
 [[Category: Worbs, M.]]
 [[Category: termination]]
 [[Category: transcription regulation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:34:27 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:01:11 2008''