1hf8: Difference between revisions
New page: left|200px<br /><applet load="1hf8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hf8, resolution 2.0Å" /> '''CALM-N N-TERMINAL DOM... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1hf8.jpg|left|200px]]<br /><applet load="1hf8" size=" | [[Image:1hf8.jpg|left|200px]]<br /><applet load="1hf8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1hf8, resolution 2.0Å" /> | caption="1hf8, resolution 2.0Å" /> | ||
'''CALM-N N-TERMINAL DOMAIN OF CLATHRIN ASSEMBLY LYMPHOID MYELOID LEUKAEMIA PROTEIN'''<br /> | '''CALM-N N-TERMINAL DOMAIN OF CLATHRIN ASSEMBLY LYMPHOID MYELOID LEUKAEMIA PROTEIN'''<br /> | ||
==Overview== | ==Overview== | ||
Adaptor protein 180 (AP180) and its homolog, clathrin assembly lymphoid | Adaptor protein 180 (AP180) and its homolog, clathrin assembly lymphoid myeloid leukemia protein (CALM), are closely related proteins that play important roles in clathrin-mediated endocytosis. Here, we present the structure of the NH2-terminal domain of CALM bound to phosphatidylinositol-4,5- bisphosphate [PtdIns(4,5)P2] via a lysine-rich motif. This motif is found in other proteins predicted to have domains of similar structure (for example, Huntingtin interacting protein 1). The structure is in part similar to the epsin NH2-terminal (ENTH) domain, but epsin lacks the PtdIns(4,5)P2-binding site. Because AP180 could bind to PtdIns(4,5)P2 and clathrin simultaneously, it may serve to tether clathrin to the membrane. This was shown by using purified components and a budding assay on preformed lipid monolayers. In the presence of AP180, clathrin lattices formed on the monolayer. When AP2 was also present, coated pits were formed. | ||
==About this Structure== | ==About this Structure== | ||
1HF8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http:// | 1HF8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HF8 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Evans, P | [[Category: Evans, P R.]] | ||
[[Category: Ford, M | [[Category: Ford, M G.J.]] | ||
[[Category: Mcmahon, H | [[Category: Mcmahon, H T.]] | ||
[[Category: alpha-alpha superhelix]] | [[Category: alpha-alpha superhelix]] | ||
[[Category: clathrin]] | [[Category: clathrin]] | ||
| Line 23: | Line 23: | ||
[[Category: triskelion]] | [[Category: triskelion]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:00:42 2008'' | ||
Revision as of 14:00, 21 February 2008
|
CALM-N N-TERMINAL DOMAIN OF CLATHRIN ASSEMBLY LYMPHOID MYELOID LEUKAEMIA PROTEIN
OverviewOverview
Adaptor protein 180 (AP180) and its homolog, clathrin assembly lymphoid myeloid leukemia protein (CALM), are closely related proteins that play important roles in clathrin-mediated endocytosis. Here, we present the structure of the NH2-terminal domain of CALM bound to phosphatidylinositol-4,5- bisphosphate [PtdIns(4,5)P2] via a lysine-rich motif. This motif is found in other proteins predicted to have domains of similar structure (for example, Huntingtin interacting protein 1). The structure is in part similar to the epsin NH2-terminal (ENTH) domain, but epsin lacks the PtdIns(4,5)P2-binding site. Because AP180 could bind to PtdIns(4,5)P2 and clathrin simultaneously, it may serve to tether clathrin to the membrane. This was shown by using purified components and a budding assay on preformed lipid monolayers. In the presence of AP180, clathrin lattices formed on the monolayer. When AP2 was also present, coated pits were formed.
About this StructureAbout this Structure
1HF8 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
ReferenceReference
Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes., Ford MG, Pearse BM, Higgins MK, Vallis Y, Owen DJ, Gibson A, Hopkins CR, Evans PR, McMahon HT, Science. 2001 Feb 9;291(5506):1051-5. PMID:11161218
Page seeded by OCA on Thu Feb 21 13:00:42 2008