1h8t: Difference between revisions
New page: left|200px<br /><applet load="1h8t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h8t, resolution 2.90Å" /> '''ECHOVIRUS 11'''<br /... |
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[[Image:1h8t.jpg|left|200px]]<br /><applet load="1h8t" size=" | [[Image:1h8t.jpg|left|200px]]<br /><applet load="1h8t" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1h8t, resolution 2.90Å" /> | caption="1h8t, resolution 2.90Å" /> | ||
'''ECHOVIRUS 11'''<br /> | '''ECHOVIRUS 11'''<br /> | ||
==Overview== | ==Overview== | ||
We have used X-ray crystallography to determine the structure of a decay | We have used X-ray crystallography to determine the structure of a decay accelerating factor (DAF)-binding, clinic-derived isolate of echovirus 11 (EV11-207). The structures of the capsid proteins closely resemble those of capsid proteins of other picornaviruses. The structure allows us to interpret a series of amino acid changes produced by passaging EV11-207 in different cell lines as highlighting the locations of multiple receptor-binding sites on the virion surface. We suggest that a DAF-binding site is located at the fivefold axes of the virion, while the binding site for a distinct but as yet unidentified receptor is located within the canyon surrounding the virion fivefold axes. | ||
==About this Structure== | ==About this Structure== | ||
1H8T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_echovirus_25 Human echovirus 25] with DOA and MYR as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1H8T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_echovirus_25 Human echovirus 25] with <scene name='pdbligand=DOA:'>DOA</scene> and <scene name='pdbligand=MYR:'>MYR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8T OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Human echovirus 25]] | [[Category: Human echovirus 25]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Brown, T | [[Category: Brown, T D.K.]] | ||
[[Category: Harley, C.]] | [[Category: Harley, C.]] | ||
[[Category: Lea, S | [[Category: Lea, S M.]] | ||
[[Category: Mckee, T.]] | [[Category: Mckee, T.]] | ||
[[Category: Stuart, A.]] | [[Category: Stuart, A.]] | ||
[[Category: Stuart, D | [[Category: Stuart, D I.]] | ||
[[Category: Williams, P | [[Category: Williams, P A.]] | ||
[[Category: DOA]] | [[Category: DOA]] | ||
[[Category: MYR]] | [[Category: MYR]] | ||
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[[Category: icosahedral virus]] | [[Category: icosahedral virus]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:58:41 2008'' | ||
Revision as of 13:58, 21 February 2008
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ECHOVIRUS 11
OverviewOverview
We have used X-ray crystallography to determine the structure of a decay accelerating factor (DAF)-binding, clinic-derived isolate of echovirus 11 (EV11-207). The structures of the capsid proteins closely resemble those of capsid proteins of other picornaviruses. The structure allows us to interpret a series of amino acid changes produced by passaging EV11-207 in different cell lines as highlighting the locations of multiple receptor-binding sites on the virion surface. We suggest that a DAF-binding site is located at the fivefold axes of the virion, while the binding site for a distinct but as yet unidentified receptor is located within the canyon surrounding the virion fivefold axes.
About this StructureAbout this Structure
1H8T is a Protein complex structure of sequences from Human echovirus 25 with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Determination of the structure of a decay accelerating factor-binding clinical isolate of echovirus 11 allows mapping of mutants with altered receptor requirements for infection., Stuart AD, McKee TA, Williams PA, Harley C, Shen S, Stuart DI, Brown TD, Lea SM, J Virol. 2002 Aug;76(15):7694-704. PMID:12097583
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