1h3h: Difference between revisions
New page: left|200px<br /><applet load="1h3h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h3h" /> '''STRUCTURAL BASIS FOR SPECIFIC RECOGNITION OF... |
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[[Image:1h3h.jpg|left|200px]]<br /><applet load="1h3h" size=" | [[Image:1h3h.jpg|left|200px]]<br /><applet load="1h3h" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''STRUCTURAL BASIS FOR SPECIFIC RECOGNITION OF AN RXXK-CONTAINING SLP-76 PEPTIDE BY THE GADS C-TERMINAL SH3 DOMAIN'''<br /> | '''STRUCTURAL BASIS FOR SPECIFIC RECOGNITION OF AN RXXK-CONTAINING SLP-76 PEPTIDE BY THE GADS C-TERMINAL SH3 DOMAIN'''<br /> | ||
==Overview== | |||
The SH3 domain, which normally recognizes proline-rich sequences, has the potential to bind motifs with an RxxK consensus. To explore this novel specificity, we have determined the solution structure of the Gads T cell adaptor C-terminal SH3 domain in complex with an RSTK-containing peptide, representing its physiological binding site on the SLP-76 docking protein. The SLP-76 peptide engages four distinct binding pockets on the surface of the Gads SH3 domain and upon binding adopts a unique structure characterized by a right-handed 3(10) helix at the RSTK locus, in contrast to the left-handed polyproline type II helix formed by canonical proline-rich SH3 ligands. The structure, and supporting mutagenesis and peptide binding data, reveal a novel mode of ligand recognition by SH3 domains. | |||
==About this Structure== | ==About this Structure== | ||
1H3H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | 1H3H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H3H OCA]. | ||
==Reference== | |||
Structural basis for specific binding of the Gads SH3 domain to an RxxK motif-containing SLP-76 peptide: a novel mode of peptide recognition., Liu Q, Berry D, Nash P, Pawson T, McGlade CJ, Li SS, Mol Cell. 2003 Feb;11(2):471-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12620234 12620234] | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Berry, D.]] | [[Category: Berry, D.]] | ||
[[Category: Li, S | [[Category: Li, S S.]] | ||
[[Category: Liu, Q.]] | [[Category: Liu, Q.]] | ||
[[Category: Mcglade, C | [[Category: Mcglade, C J.]] | ||
[[Category: Nash, P.]] | [[Category: Nash, P.]] | ||
[[Category: Pawson, T.]] | [[Category: Pawson, T.]] | ||
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[[Category: t-cell signaling]] | [[Category: t-cell signaling]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:57:00 2008'' | ||
