1h0q: Difference between revisions

Revision as of 13:56, 21 February 2008

NMR SOLUTION STRUCTURE OF A FULLY MODIFIED LOCKED NUCLEIC ACID (LNA) HYBRIDIZED TO RNA

OverviewOverview

LNA is a bicyclic nucleic acid analogue that contains one or more 2'-O,4'-C methylene linkage(s), which effectively locks the furanose ring in a C3'-endo conformation. We report here the NMR solution structure of a nonamer LNA:RNA hybrid and a structural characterization of a nonamer LNA:DNA hybrid, where the LNA strands are composed entirely of LNA nucleotides. This is the first structural characterization of fully modified LNA oligonucleotides. The high-resolution structure reveals that the LNA:RNA hybrid adopts an almost canonical A-type duplex morphology. The helix axis is almost straight and the duplex geometry is regular. This shows that fully modified LNA oligomers can hybridize with complementary RNA and form duplexes within the Watson-Crick framework. The LNA:DNA hybrid structurally resembles an RNA:DNA hybrid as shown by determination of deoxyribose sugar puckers and analysis of NOESY NMR spectra.

About this StructureAbout this Structure

1H0Q is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

ReferenceReference

NMR studies of fully modified locked nucleic acid (LNA) hybrids: solution structure of an LNA:RNA hybrid and characterization of an LNA:DNA hybrid., Nielsen KE, Rasmussen J, Kumar R, Wengel J, Jacobsen JP, Petersen M, Bioconjug Chem. 2004 May-Jun;15(3):449-57. PMID:15149171

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-[[Image:1h0q.gif|left|200px]]<br /><applet load="1h0q" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1h0q.gif|left|200px]]<br /><applet load="1h0q" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1h0q" />
 '''NMR SOLUTION STRUCTURE OF A FULLY MODIFIED LOCKED NUCLEIC ACID (LNA) HYBRIDIZED TO RNA'''<br />
 ==Overview==
-LNA is a bicyclic nucleic acid analogue that contains one or more, 2'-O,4'-C methylene linkage(s), which effectively locks the furanose ring, in a C3'-endo conformation. We report here the NMR solution structure of a, nonamer LNA:RNA hybrid and a structural characterization of a nonamer, LNA:DNA hybrid, where the LNA strands are composed entirely of LNA, nucleotides. This is the first structural characterization of fully, modified LNA oligonucleotides. The high-resolution structure reveals that, the LNA:RNA hybrid adopts an almost canonical A-type duplex morphology., The helix axis is almost straight and the duplex geometry is regular. This, shows that fully modified LNA oligomers can hybridize with complementary, RNA and form duplexes within the Watson-Crick framework. The LNA:DNA, hybrid structurally resembles an RNA:DNA hybrid as shown by determination, of deoxyribose sugar puckers and analysis of NOESY NMR spectra.
+LNA is a bicyclic nucleic acid analogue that contains one or more 2'-O,4'-C methylene linkage(s), which effectively locks the furanose ring in a C3'-endo conformation. We report here the NMR solution structure of a nonamer LNA:RNA hybrid and a structural characterization of a nonamer LNA:DNA hybrid, where the LNA strands are composed entirely of LNA nucleotides. This is the first structural characterization of fully modified LNA oligonucleotides. The high-resolution structure reveals that the LNA:RNA hybrid adopts an almost canonical A-type duplex morphology. The helix axis is almost straight and the duplex geometry is regular. This shows that fully modified LNA oligomers can hybridize with complementary RNA and form duplexes within the Watson-Crick framework. The LNA:DNA hybrid structurally resembles an RNA:DNA hybrid as shown by determination of deoxyribose sugar puckers and analysis of NOESY NMR spectra.
 ==About this Structure==
-H0Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H0Q OCA].
+H0Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H0Q OCA].
 ==Reference==
 NMR studies of fully modified locked nucleic acid (LNA) hybrids: solution structure of an LNA:RNA hybrid and characterization of an LNA:DNA hybrid., Nielsen KE, Rasmussen J, Kumar R, Wengel J, Jacobsen JP, Petersen M, Bioconjug Chem. 2004 May-Jun;15(3):449-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15149171 15149171]
 [[Category: Single protein]]
-[[Category: Jacobsen, J.P.]]
+[[Category: Jacobsen, J P.]]
 [[Category: Kumar, R.]]
-[[Category: Nielsen, K.E.]]
+[[Category: Nielsen, K E.]]
 [[Category: Petersen, M.]]
 [[Category: Rasmussen, J.]]
@@ Line 24: / Line 24: @@
 [[Category: rna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:03:40 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:56:07 2008''