1gye: Difference between revisions
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==Overview== | ==Overview== | ||
Cellvibrio japonicus arabinanase Arb43A hydrolyzes the alpha-1,5-linked | Cellvibrio japonicus arabinanase Arb43A hydrolyzes the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The three-dimensional structure of Arb43A, determined at 1.9 A resolution, reveals a five-bladed beta-propeller fold. Arb43A is the first enzyme known to display this topology. A long V-shaped surface groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. Three carboxylates deep in the active site groove provide the general acid and base components for glycosidic bond hydrolysis with inversion of anomeric configuration. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Cellvibrio japonicus]] | [[Category: Cellvibrio japonicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Charnock, S | [[Category: Charnock, S J.]] | ||
[[Category: Davies, G | [[Category: Davies, G J.]] | ||
[[Category: Dodson, E | [[Category: Dodson, E J.]] | ||
[[Category: Gilbert, H | [[Category: Gilbert, H J.]] | ||
[[Category: Mckie, V | [[Category: Mckie, V A.]] | ||
[[Category: Nurizzo, D.]] | [[Category: Nurizzo, D.]] | ||
[[Category: Roberts, S | [[Category: Roberts, S M.]] | ||
[[Category: Taylor, E | [[Category: Taylor, E J.]] | ||
[[Category: Turkenburg, J | [[Category: Turkenburg, J P.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
[[Category: arabinanase]] | [[Category: arabinanase]] | ||
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[[Category: pseudomonas]] | [[Category: pseudomonas]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:55:23 2008'' | ||
Revision as of 13:55, 21 February 2008
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STRUCTURE OF CELLVIBRIO CELLULOSA ALPHA-L-ARABINANASE COMPLEXED WITH ARABINOHEXAOSE
OverviewOverview
Cellvibrio japonicus arabinanase Arb43A hydrolyzes the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The three-dimensional structure of Arb43A, determined at 1.9 A resolution, reveals a five-bladed beta-propeller fold. Arb43A is the first enzyme known to display this topology. A long V-shaped surface groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. Three carboxylates deep in the active site groove provide the general acid and base components for glycosidic bond hydrolysis with inversion of anomeric configuration.
About this StructureAbout this Structure
1GYE is a Single protein structure of sequence from Cellvibrio japonicus with as ligand. Active as Arabinan endo-1,5-alpha-L-arabinosidase, with EC number 3.2.1.99 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold., Nurizzo D, Turkenburg JP, Charnock SJ, Roberts SM, Dodson EJ, McKie VA, Taylor EJ, Gilbert HJ, Davies GJ, Nat Struct Biol. 2002 Sep;9(9):665-8. PMID:12198486
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