1gl2: Difference between revisions

Revision as of 13:51, 21 February 2008

CRYSTAL STRUCTURE OF AN ENDOSOMAL SNARE CORE COMPLEX

OverviewOverview

SNARE proteins are crucial for intracellular membrane fusion in all eukaryotes. These proteins assemble into tight complexes that connect membranes and may induce fusion. The crystal structure of the neuronal core complex is represented by an unusually long bundle of four alpha-helices connected by 16 layers of mostly hydrophobic amino acids. Here we report the 1.9 A resolution crystal structure of an endosomal SNARE core complex containing four SNAREs: syntaxin 7, syntaxin 8, vti1b and endobrevin/VAMP-8. Despite limited sequence homology, the helix alignment and the layer structure of the endosomal complex are remarkably similar to those of the neuronal complex. However, subtle variations are evident that characterize different SNARE subfamilies. We conclude that the structure of the SNARE core complex is an evolutionarily conserved hallmark of all SNARE complexes and is intimately associated with the general role of SNAREs in membrane fusion.

About this StructureAbout this Structure

1GL2 is a Protein complex structure of sequences from Mus musculus and Rattus norvegicus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs., Antonin W, Fasshauer D, Becker S, Jahn R, Schneider TR, Nat Struct Biol. 2002 Feb;9(2):107-11. PMID:11786915

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OCA

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-[[Image:1gl2.jpg|left|200px]]<br /><applet load="1gl2" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1gl2, resolution 1.90&Aring;" />
 '''CRYSTAL STRUCTURE OF AN ENDOSOMAL SNARE CORE COMPLEX'''<br />
 ==Overview==
-SNARE proteins are crucial for intracellular membrane fusion in all, eukaryotes. These proteins assemble into tight complexes that connect, membranes and may induce fusion. The crystal structure of the neuronal, core complex is represented by an unusually long bundle of four, alpha-helices connected by 16 layers of mostly hydrophobic amino acids., Here we report the 1.9 A resolution crystal structure of an endosomal, SNARE core complex containing four SNAREs: syntaxin 7, syntaxin 8, vti1b, and endobrevin/VAMP-8. Despite limited sequence homology, the helix, alignment and the layer structure of the endosomal complex are remarkably, similar to those of the neuronal complex. However, subtle variations are, evident that characterize different SNARE subfamilies. We conclude that, the structure of the SNARE core complex is an evolutionarily conserved, hallmark of all SNARE complexes and is intimately associated with the, general role of SNAREs in membrane fusion.
+SNARE proteins are crucial for intracellular membrane fusion in all eukaryotes. These proteins assemble into tight complexes that connect membranes and may induce fusion. The crystal structure of the neuronal core complex is represented by an unusually long bundle of four alpha-helices connected by 16 layers of mostly hydrophobic amino acids. Here we report the 1.9 A resolution crystal structure of an endosomal SNARE core complex containing four SNAREs: syntaxin 7, syntaxin 8, vti1b and endobrevin/VAMP-8. Despite limited sequence homology, the helix alignment and the layer structure of the endosomal complex are remarkably similar to those of the neuronal complex. However, subtle variations are evident that characterize different SNARE subfamilies. We conclude that the structure of the SNARE core complex is an evolutionarily conserved hallmark of all SNARE complexes and is intimately associated with the general role of SNAREs in membrane fusion.
 ==About this Structure==
-GL2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GL2 OCA].
+GL2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GL2 OCA].
 ==Reference==
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 [[Category: Becker, S.]]
 [[Category: Jahn, R.]]
-[[Category: Schneider, T.R.]]
+[[Category: Schneider, T R.]]
 [[Category: coiled coil]]
 [[Category: membrane fusion protein complex]]
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 [[Category: transport]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:08:05 2007''
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