1ohb: Difference between revisions
New page: left|200px<br /> <applet load="1ohb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ohb, resolution 1.90Å" /> '''ACETYLGLUTAMATE KIN... |
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==About this Structure== | ==About this Structure== | ||
1OHB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SO4, ACT and ADP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OHB OCA]]. | 1OHB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with SO4, ACT and ADP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OHB OCA]]. | ||
==Reference== | ==Reference== | ||
The course of phosphorus in the reaction of N-acetyl-L-glutamate kinase, determined from the structures of crystalline complexes, including a complex with an AlF(4)(-) transition state mimic., Gil-Ortiz F, Ramon-Maiques S, Fita I, Rubio V, J Mol Biol. 2003 Aug 1;331(1):231-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12875848 12875848] | The course of phosphorus in the reaction of N-acetyl-L-glutamate kinase, determined from the structures of crystalline complexes, including a complex with an AlF(4)(-) transition state mimic., Gil-Ortiz F, Ramon-Maiques S, Fita I, Rubio V, J Mol Biol. 2003 Aug 1;331(1):231-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12875848 12875848] | ||
[[Category: Acetylglutamate kinase]] | |||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: x-ray diffraction]] | [[Category: x-ray diffraction]] | ||
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Revision as of 15:03, 30 October 2007
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ACETYLGLUTAMATE KINASE FROM ESCHERICHIA COLI COMPLEXED WITH ADP AND SULPHATE
OverviewOverview
N-Acetyl-L-glutamate kinase (NAGK), the structural paradigm of the enzymes, of the amino acid kinase family, catalyzes the phosphorylation of the, gamma-COO(-) group of N-acetyl-L-glutamate (NAG) by ATP. We determine here, the crystal structures of NAGK complexes with MgADP, NAG and the, transition-state analog AlF(4)(-); with MgADP and NAG; and with ADP and, SO(4)(2-). Comparison of these structures with that of the MgAMPPNP-NAG, complex allows to delineate three successive steps during phosphoryl, transfer: at the beginning, when the attacking and leaving O atoms and the, P atom are imperfectly aligned and the distance between the attacking O, atom and the P atom is 2.8A; midway, at the bipyramidal intermediate, with, nearly perfect alignment and a distance of 2.3A; and, when the ... [(full description)]
About this StructureAbout this Structure
1OHB is a [Single protein] structure of sequence from [Escherichia coli] with SO4, ACT and ADP as [ligands]. Active as [Acetylglutamate kinase], with EC number [2.7.2.8]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
The course of phosphorus in the reaction of N-acetyl-L-glutamate kinase, determined from the structures of crystalline complexes, including a complex with an AlF(4)(-) transition state mimic., Gil-Ortiz F, Ramon-Maiques S, Fita I, Rubio V, J Mol Biol. 2003 Aug 1;331(1):231-44. PMID:12875848
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