1bko: Difference between revisions
New page: left|200px<br /> <applet load="1bko" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bko, resolution 2.75Å" /> '''THERMOSTABLE THYMID... |
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==About this Structure== | ==About this Structure== | ||
1BKO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BKO OCA]]. | 1BKO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]]. Active as [[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45]]. Structure known Active Sites: CA1, CA2, CA3 and CA4. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BKO OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thymidylate synthase]] | |||
[[Category: Santi, D.V.]] | [[Category: Santi, D.V.]] | ||
[[Category: Schellenberger, U.]] | [[Category: Schellenberger, U.]] | ||
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[[Category: methyltransferase]] | [[Category: methyltransferase]] | ||
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Revision as of 15:02, 30 October 2007
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THERMOSTABLE THYMIDYLATE SYNTHASE A FROM BACILLUS SUBTILIS
OverviewOverview
Unlike all other organisms studied to date, Bacillus subtilis expresses, two different thymidylate synthases: bsTS-A and bsTS-B. bsTS-A displays, enhanced enzymatic and structural thermal stability uncharacteristic of, most TSs. Despite the high level of TS conservation across most species, bsTS-A shares low sequence identity (<40%) with the majority of TSs from, other organisms. This TS and the TSs from Lactococcus lactis and phage, Phi3T-to which it is most similar-have been of interest for some time, since, by structure-based sequence alignment, they appear to lack several, key residues shown by mutagenesis to be essential to enzymatic function, [Greene, P. J., Yu, P. L., Zhao, J., Schiffer, C. A., and Santi, D. (1994), Protein Sci. 3, 1114-6]. In addition, bsTS-A demonstrates ... [(full description)]
About this StructureAbout this Structure
1BKO is a [Single protein] structure of sequence from [Bacillus subtilis]. Active as [Thymidylate synthase], with EC number [2.1.1.45]. Structure known Active Sites: CA1, CA2, CA3 and CA4. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structures of a unique thermal-stable thymidylate synthase from Bacillus subtilis., Stout TJ, Schellenberger U, Santi DV, Stroud RM, Biochemistry. 1998 Oct 20;37(42):14736-47. PMID:9778348
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