1fos: Difference between revisions

Revision as of 13:40, 21 February 2008

TWO HUMAN C-FOS:C-JUN:DNA COMPLEXES

OverviewOverview

The Fos and Jun families of eukaryotic transcription factors heterodimerize to form complexes capable of binding 5'-TGAGTCA-3' DNA elements. We have determined the X-ray crystal structure of a heterodimer of the bZIP regions of c-Fos and c-Jun bound to DNA. Both subunits form continuous alpha-helices. The carboxy-terminal regions form an asymmetric coiled-coil, and the amino-terminal regions make base-specific contacts with DNA in the major groove. Comparison of the two crystallographically distinct protein-DNA complexes show that the coiled-coil is flexibly joined to the basic regions and that the Fos-Jun heterodimer does not recognize the asymmetric 5'-TGAGTCA-3' recognition element in a unique orientation. There is an extensive network of electrostatic interactions between subunits within the coiled-coil, consistent with proposals that these interactions determine preferential formation of the heterodimer over either of the homodimers.

About this StructureAbout this Structure

1FOS is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA., Glover JN, Harrison SC, Nature. 1995 Jan 19;373(6511):257-61. PMID:7816143

Page seeded by OCA on Thu Feb 21 12:40:54 2008

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OCA

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-[[Image:1fos.gif|left|200px]]<br />
+[[Image:1fos.gif|left|200px]]<br /><applet load="1fos" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1fos" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1fos, resolution 3.050&Aring;" />
 '''TWO HUMAN C-FOS:C-JUN:DNA COMPLEXES'''<br />
 ==Overview==
-The Fos and Jun families of eukaryotic transcription factors, heterodimerize to form complexes capable of binding 5'-TGAGTCA-3' DNA, elements. We have determined the X-ray crystal structure of a heterodimer, of the bZIP regions of c-Fos and c-Jun bound to DNA. Both subunits form, continuous alpha-helices. The carboxy-terminal regions form an asymmetric, coiled-coil, and the amino-terminal regions make base-specific contacts, with DNA in the major groove. Comparison of the two crystallographically, distinct protein-DNA complexes show that the coiled-coil is flexibly, joined to the basic regions and that the Fos-Jun heterodimer does not, recognize the asymmetric 5'-TGAGTCA-3' recognition element in a unique, orientation. There is an extensive network of electrostatic interactions, between subunits within the coiled-coil, consistent with proposals that, these interactions determine preferential formation of the heterodimer, over either of the homodimers.
+The Fos and Jun families of eukaryotic transcription factors heterodimerize to form complexes capable of binding 5'-TGAGTCA-3' DNA elements. We have determined the X-ray crystal structure of a heterodimer of the bZIP regions of c-Fos and c-Jun bound to DNA. Both subunits form continuous alpha-helices. The carboxy-terminal regions form an asymmetric coiled-coil, and the amino-terminal regions make base-specific contacts with DNA in the major groove. Comparison of the two crystallographically distinct protein-DNA complexes show that the coiled-coil is flexibly joined to the basic regions and that the Fos-Jun heterodimer does not recognize the asymmetric 5'-TGAGTCA-3' recognition element in a unique orientation. There is an extensive network of electrostatic interactions between subunits within the coiled-coil, consistent with proposals that these interactions determine preferential formation of the heterodimer over either of the homodimers.
 ==About this Structure==
-FOS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FOS OCA].
+FOS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOS OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
-[[Category: Glover, J.N.M.]]
+[[Category: Glover, J N.M.]]
-[[Category: Harrison, S.C.]]
+[[Category: Harrison, S C.]]
 [[Category: coiled-coil]]
 [[Category: dna-binding protein]]
 [[Category: heterodimer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:55:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:40:54 2008''