1fdt: Difference between revisions

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HUMAN 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE 1 COMPLEXED WITH ESTRADIOL AND NADP+

OverviewOverview

BACKGROUND: The steroid hormone 17beta-estradiol is important in the genesis and development of human breast cancer. Its intracellular concentration is regulated by 17beta-hydroxysteroid dehydrogenase, which catalyzes the reversible reduction of estrone to 17beta-estradiol. This enzyme is thus an important target for inhibitor design. The precise localization and orientation of the substrate and cofactor in the active site is of paramount importance for the design of such inhibitors, and for an understanding of the catalytic mechanism. RESULTS: The structure of recombinant human 17beta-hydroxysteroid dehydrogenase of type 1 (17beta-HSD1) in complex with estradiol at room temperature has been determined at 1.7 A resolution, and a ternary 17betaHSD1-estradiol-NADP+ complex at -150 degrees C has been solved and refined at 2.20 A resolution. The structures show that estradiol interacts with the enzyme through three hydrogen bonds (involving side chains of Ser142, Tyr155 and His221), and hydrophobic interactions between the core of the steroid and nine other residues. The NADP+ molecule binds in an extended conformation, with the nicotinamide ring close to the estradiol molecule. CONCLUSIONS: From the structure of the complex of the enzyme with the substrate and cofactor of the oxidation reaction, the orientation of the substrates for the reduction reaction can be deduced with confidence. A triangular hydrogen-bond network between Tyr155, Ser142 and O17 from estradiol probably facilitates the deprotonation of the reactive tyrosine, while the conserved Lys159 appears not to be directly involved in catalysis. Both the steroid-binding site and the NADPH-binding site can be proposed as targets for the design of inhibitors.

About this StructureAbout this Structure

1FDT is a Single protein structure of sequence from Homo sapiens with , and as ligands. Active as Estradiol 17-beta-dehydrogenase, with EC number 1.1.1.62 Full crystallographic information is available from OCA.

ReferenceReference

The structure of a complex of human 17beta-hydroxysteroid dehydrogenase with estradiol and NADP+ identifies two principal targets for the design of inhibitors., Breton R, Housset D, Mazza C, Fontecilla-Camps JC, Structure. 1996 Aug 15;4(8):905-15. PMID:8805577

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-[[Image:1fdt.gif|left|200px]]<br />
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 caption="1fdt, resolution 2.2&Aring;" />
 '''HUMAN 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE 1 COMPLEXED WITH ESTRADIOL AND NADP+'''<br />
 ==Overview==
-BACKGROUND: The steroid hormone 17beta-estradiol is important in the, genesis and development of human breast cancer. Its intracellular, concentration is regulated by 17beta-hydroxysteroid dehydrogenase, which, catalyzes the reversible reduction of estrone to 17beta-estradiol. This, enzyme is thus an important target for inhibitor design. The precise, localization and orientation of the substrate and cofactor in the active, site is of paramount importance for the design of such inhibitors, and for, an understanding of the catalytic mechanism. RESULTS: The structure of, recombinant human 17beta-hydroxysteroid dehydrogenase of type 1, (17beta-HSD1) in complex with estradiol at room temperature has been, determined at 1.7 A resolution, and a ternary 17betaHSD1-estradiol-NADP+, complex at -150 degrees C has been solved and refined at 2.20 A, resolution. The structures show that estradiol interacts with the enzyme, through three hydrogen bonds (involving side chains of Ser142, Tyr155 and, His221), and hydrophobic interactions between the core of the steroid and, nine other residues. The NADP+ molecule binds in an extended conformation, with the nicotinamide ring close to the estradiol molecule. CONCLUSIONS:, From the structure of the complex of the enzyme with the substrate and, cofactor of the oxidation reaction, the orientation of the substrates for, the reduction reaction can be deduced with confidence. A triangular, hydrogen-bond network between Tyr155, Ser142 and O17 from estradiol, probably facilitates the deprotonation of the reactive tyrosine, while the, conserved Lys159 appears not to be directly involved in catalysis. Both, the steroid-binding site and the NADPH-binding site can be proposed as, targets for the design of inhibitors.
+BACKGROUND: The steroid hormone 17beta-estradiol is important in the genesis and development of human breast cancer. Its intracellular concentration is regulated by 17beta-hydroxysteroid dehydrogenase, which catalyzes the reversible reduction of estrone to 17beta-estradiol. This enzyme is thus an important target for inhibitor design. The precise localization and orientation of the substrate and cofactor in the active site is of paramount importance for the design of such inhibitors, and for an understanding of the catalytic mechanism. RESULTS: The structure of recombinant human 17beta-hydroxysteroid dehydrogenase of type 1 (17beta-HSD1) in complex with estradiol at room temperature has been determined at 1.7 A resolution, and a ternary 17betaHSD1-estradiol-NADP+ complex at -150 degrees C has been solved and refined at 2.20 A resolution. The structures show that estradiol interacts with the enzyme through three hydrogen bonds (involving side chains of Ser142, Tyr155 and His221), and hydrophobic interactions between the core of the steroid and nine other residues. The NADP+ molecule binds in an extended conformation, with the nicotinamide ring close to the estradiol molecule. CONCLUSIONS: From the structure of the complex of the enzyme with the substrate and cofactor of the oxidation reaction, the orientation of the substrates for the reduction reaction can be deduced with confidence. A triangular hydrogen-bond network between Tyr155, Ser142 and O17 from estradiol probably facilitates the deprotonation of the reactive tyrosine, while the conserved Lys159 appears not to be directly involved in catalysis. Both the steroid-binding site and the NADPH-binding site can be proposed as targets for the design of inhibitors.
 ==About this Structure==
-FDT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4, EST and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Estradiol_17-beta-dehydrogenase Estradiol 17-beta-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.62 1.1.1.62] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FDT OCA].
+FDT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=EST:'>EST</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Estradiol_17-beta-dehydrogenase Estradiol 17-beta-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.62 1.1.1.62] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FDT OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Breton, R.]]
-[[Category: Fontecilla-Camps, J.C.]]
+[[Category: Fontecilla-Camps, J C.]]
 [[Category: Housset, D.]]
 [[Category: Mazza, C.]]
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 [[Category: dehydrogenase]]
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