1fdo: Difference between revisions
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==Overview== | ==Overview== | ||
Formate dehydrogenase H from Escherichia coli contains selenocysteine | Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and catalyzes the two-electron oxidation of formate to carbon dioxide. The crystal structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate dehydrogenase H (with and without bound inhibitor) and the reduced [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain alphabeta structure with the molybdenum directly coordinated to selenium and both MGD cofactors. These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Formate dehydrogenase]] | [[Category: Formate dehydrogenase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Boyington, J | [[Category: Boyington, J C.]] | ||
[[Category: Sun, P | [[Category: Sun, P D.]] | ||
[[Category: 6MO]] | [[Category: 6MO]] | ||
[[Category: MGD]] | [[Category: MGD]] | ||
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[[Category: selenocysteine]] | [[Category: selenocysteine]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:37:36 2008'' | ||
Revision as of 13:37, 21 February 2008
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OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI
OverviewOverview
Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and catalyzes the two-electron oxidation of formate to carbon dioxide. The crystal structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate dehydrogenase H (with and without bound inhibitor) and the reduced [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain alphabeta structure with the molybdenum directly coordinated to selenium and both MGD cofactors. These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer.
About this StructureAbout this Structure
1FDO is a Single protein structure of sequence from Escherichia coli with , and as ligands. Active as Formate dehydrogenase, with EC number 1.2.1.2 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:9036855
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