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-[[Image:1fc8.gif|left|200px]]<br /><applet load="1fc8" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1fc8.gif|left|200px]]<br /><applet load="1fc8" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1fc8" />
 '''NMR SOLUTION STRUCTURE OF A CHIMERIC OLIGONUCLEOTIDE HAIRPIN R(GGAC)D(TTCG)2'F-A(GTCC)'''<br />
 ==Overview==
-The first structure of a 2'-deoxy-2'-fluoro-D-arabinose nucleic acid, (2'F-ANA)/RNA duplex is presented. We report the structural, characterization by NMR spectroscopy of a small hybrid hairpin, r(GGAC)d(TTCG)2'F-a(GTCC), containing a 2'F-ANA/RNA stem and a, four-residue DNA loop. Complete (1)H, (13)C, (19)F, and (31)P resonance, assignments, scalar coupling constants, and NOE constraints were obtained, from homonuclear and heteronuclear 2D spectra. In the chimeric duplex, the, RNA strand adopts a classic A-form structure having C3' endo sugar, puckers. The 2'F-ANA strand is neither A-form nor B-form and contains O4', endo sugar puckers. This contrasts strongly with the dynamic sugar, conformations previously observed in the DNA strands of DNA/RNA hybrid, duplexes. Structural parameters for the duplex, such as minor groove, width, x-displacement, and inclination, were intermediate between those of, A-form and B-form duplexes and similar to those of DNA/RNA duplexes. These, results rationalize the enhanced stability of 2'F-ANA/RNA duplexes and, their ability to elicit RNase H activity. The results are relevant for the, design of new antisense drugs based on sugar-modified nucleic acids.
+The first structure of a 2'-deoxy-2'-fluoro-D-arabinose nucleic acid (2'F-ANA)/RNA duplex is presented. We report the structural characterization by NMR spectroscopy of a small hybrid hairpin, r(GGAC)d(TTCG)2'F-a(GTCC), containing a 2'F-ANA/RNA stem and a four-residue DNA loop. Complete (1)H, (13)C, (19)F, and (31)P resonance assignments, scalar coupling constants, and NOE constraints were obtained from homonuclear and heteronuclear 2D spectra. In the chimeric duplex, the RNA strand adopts a classic A-form structure having C3' endo sugar puckers. The 2'F-ANA strand is neither A-form nor B-form and contains O4' endo sugar puckers. This contrasts strongly with the dynamic sugar conformations previously observed in the DNA strands of DNA/RNA hybrid duplexes. Structural parameters for the duplex, such as minor groove width, x-displacement, and inclination, were intermediate between those of A-form and B-form duplexes and similar to those of DNA/RNA duplexes. These results rationalize the enhanced stability of 2'F-ANA/RNA duplexes and their ability to elicit RNase H activity. The results are relevant for the design of new antisense drugs based on sugar-modified nucleic acids.
 ==About this Structure==
-FC8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FC8 OCA].
+FC8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FC8 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Gehring, K.]]
-[[Category: Trempe, J.F.]]
+[[Category: Trempe, J F.]]
 [[Category: 2'f-arabinonucleic acid]]
 [[Category: dna]]
@@ Line 20: / Line 20: @@
 [[Category: rna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:37:54 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:37:10 2008''