1f5q: Difference between revisions
New page: left|200px<br /> <applet load="1f5q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f5q, resolution 2.50Å" /> '''CRYSTAL STRUCTURE O... |
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[[Image:1f5q.gif|left|200px]]<br /> | [[Image:1f5q.gif|left|200px]]<br /><applet load="1f5q" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1f5q" size=" | |||
caption="1f5q, resolution 2.50Å" /> | caption="1f5q, resolution 2.50Å" /> | ||
'''CRYSTAL STRUCTURE OF MURINE GAMMA HERPESVIRUS CYCLIN COMPLEXED TO HUMAN CYCLIN DEPENDENT KINASE 2'''<br /> | '''CRYSTAL STRUCTURE OF MURINE GAMMA HERPESVIRUS CYCLIN COMPLEXED TO HUMAN CYCLIN DEPENDENT KINASE 2'''<br /> | ||
==Overview== | ==Overview== | ||
Several gamma-herpesviruses encode proteins related to the mammalian | Several gamma-herpesviruses encode proteins related to the mammalian cyclins, regulatory subunits of cyclin-dependent kinases (cdks) essential for cell cycle progression. We report a 2.5 A crystal structure of a full-length oncogenic viral cyclin from gamma-herpesvirus 68 complexed with cdk2. The viral cyclin binds cdk2 with an orientation different from cyclin A and makes several novel interactions at the interface, yet it activates cdk2 by triggering conformational changes similar to cyclin A. Sequences within the viral cyclin N-terminus lock part of the cdk2 T-loop within the core of the complex. These sequences and others are conserved amongst the viral and cellular D-type cyclins, suggesting that this structure has wider implications for other cyclin-cdk complexes. The observed resistance of this viral cyclin-cdk complex to inhibition by the p27(KIP:) cdk inhibitor is explained by sequence and conformational variation in the cyclin rendering the p27(KIP:)-binding site on the cyclin subunit non-functional. | ||
==About this Structure== | ==About this Structure== | ||
1F5Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Murid_herpesvirus_1 Murid herpesvirus 1] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1F5Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Murid_herpesvirus_1 Murid herpesvirus 1] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5Q OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Murid herpesvirus 1]] | [[Category: Murid herpesvirus 1]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Card, G | [[Category: Card, G L.]] | ||
[[Category: Jones, N.]] | [[Category: Jones, N.]] | ||
[[Category: Knowles, P.]] | [[Category: Knowles, P.]] | ||
[[Category: Laman, H.]] | [[Category: Laman, H.]] | ||
[[Category: McDonald, N | [[Category: McDonald, N Q.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
[[Category: cyclin dependent kinase. protein/protein complex]] | [[Category: cyclin dependent kinase. protein/protein complex]] | ||
[[Category: herpesviral cyclin]] | [[Category: herpesviral cyclin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:35:30 2008'' | ||
