1etj: Difference between revisions
New page: left|200px<br /><applet load="1etj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1etj, resolution 2.3Å" /> '''AZURIN MUTANT WITH ME... |
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[[Image:1etj.gif|left|200px]]<br /><applet load="1etj" size=" | [[Image:1etj.gif|left|200px]]<br /><applet load="1etj" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1etj, resolution 2.3Å" /> | caption="1etj, resolution 2.3Å" /> | ||
'''AZURIN MUTANT WITH MET 121 REPLACED BY GLU'''<br /> | '''AZURIN MUTANT WITH MET 121 REPLACED BY GLU'''<br /> | ||
==Overview== | ==Overview== | ||
The Met121Glu azurin mutant has been crystallized and the structure | The Met121Glu azurin mutant has been crystallized and the structure determined at a resolution of 2.3 A. In the crystal structure a carboxyl oxygen of Met121Glu is coordinated to the metal at a distance of 2.2 A. Single-crystal resonance Raman spectroscopy was used to show that the glutamic acid residue in the copper site was in the protonated state. Titration of this residue gives rise to a number of unusual, pH-dependent properties: as the pH is increased from 4 to 8, the S(Cys)-Cu ligand-to-metal charge transfer bands are blue shifted and their intensity ratio is reversed, the EPR signal changes from type 1 copper to a new form of protein-bound copper, and the redox potential changes from 370 to 180 mV. The spectroscopic changes in this pH interval are consistent with a two-state model. From the pH dependence of the optical and EPR spectra, pKa = 5.0 for the glutamic acid in the oxidized protein was determined. | ||
==About this Structure== | ==About this Structure== | ||
1ETJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1ETJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETJ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bonander, N.]] | [[Category: Bonander, N.]] | ||
[[Category: Karlsson, B | [[Category: Karlsson, B G.]] | ||
[[Category: Langer, V.]] | [[Category: Langer, V.]] | ||
[[Category: Nar, H.]] | [[Category: Nar, H.]] | ||
[[Category: Sanders-Loehr, J.]] | [[Category: Sanders-Loehr, J.]] | ||
[[Category: Sjolin, L.]] | [[Category: Sjolin, L.]] | ||
[[Category: Tsai, L | [[Category: Tsai, L C.]] | ||
[[Category: CU]] | [[Category: CU]] | ||
[[Category: copper]] | [[Category: copper]] | ||
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[[Category: periplasmic]] | [[Category: periplasmic]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:31:22 2008'' | ||
Revision as of 13:31, 21 February 2008
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AZURIN MUTANT WITH MET 121 REPLACED BY GLU
OverviewOverview
The Met121Glu azurin mutant has been crystallized and the structure determined at a resolution of 2.3 A. In the crystal structure a carboxyl oxygen of Met121Glu is coordinated to the metal at a distance of 2.2 A. Single-crystal resonance Raman spectroscopy was used to show that the glutamic acid residue in the copper site was in the protonated state. Titration of this residue gives rise to a number of unusual, pH-dependent properties: as the pH is increased from 4 to 8, the S(Cys)-Cu ligand-to-metal charge transfer bands are blue shifted and their intensity ratio is reversed, the EPR signal changes from type 1 copper to a new form of protein-bound copper, and the redox potential changes from 370 to 180 mV. The spectroscopic changes in this pH interval are consistent with a two-state model. From the pH dependence of the optical and EPR spectra, pKa = 5.0 for the glutamic acid in the oxidized protein was determined.
About this StructureAbout this Structure
1ETJ is a Single protein structure of sequence from Pseudomonas aeruginosa with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
X-ray structure determination and characterization of the Pseudomonas aeruginosa azurin mutant Met121Glu., Karlsson BG, Tsai LC, Nar H, Sanders-Loehr J, Bonander N, Langer V, Sjolin L, Biochemistry. 1997 Apr 8;36(14):4089-95. PMID:9100002
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