1w9b: Difference between revisions
New page: left|200px<br /> <applet load="1w9b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w9b, resolution 1.7Å" /> '''S. ALBA MYROSINASE I... |
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==About this Structure== | ==About this Structure== | ||
1W9B is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba]] with NAG, ZN, SO4, CGT and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.3.1 3.2.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W9B OCA]]. | 1W9B is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba]] with NAG, ZN, SO4, CGT and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_3.2.1.147 Transferred entry: 3.2.1.147]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.3.1 3.2.3.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W9B OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: Sinapis alba]] | [[Category: Sinapis alba]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Transferred entry: 3.2.1.147]] | |||
[[Category: Arzt, S.]] | [[Category: Arzt, S.]] | ||
[[Category: Bourderioux, A.]] | [[Category: Bourderioux, A.]] | ||
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[[Category: thiohydroximate]] | [[Category: thiohydroximate]] | ||
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Revision as of 14:52, 30 October 2007
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S. ALBA MYROSINASE IN COMPLEX WITH CARBA-GLUCOTROPAEOLIN
OverviewOverview
Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to, hydrolyse glucosinolates, a unique family of molecules bearing an anomeric, O-sulfated thiohydroximate function. Non-hydrolysable myrosinase, inhibitors have been devised and studied for their biological interaction., Diverse modifications of the O-sulfate moiety did not result in a, significant inhibitory effect, whereas replacing the D-glucopyrano residue, by its carba-analogue allowed inhibition to take place. X-Ray experiments, carried out after soaking allowed for the first time inclusion of a, non-hydrolysable inhibitor inside the enzymatic pocket. Structural tuning, of the aglycon part in its pocket is being used as a guide for the, development of simplified and more potent inhibitors.
About this StructureAbout this Structure
1W9B is a [Single protein] structure of sequence from [Sinapis alba] with NAG, ZN, SO4, CGT and GOL as [ligands]. Active as [Transferred entry: 3.2.1.147], with EC number [3.2.3.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
The glucosinolate-myrosinase system. New insights into enzyme-substrate interactions by use of simplified inhibitors., Bourderioux A, Lefoix M, Gueyrard D, Tatibouet A, Cottaz S, Arzt S, Burmeister WP, Rollin P, Org Biomol Chem. 2005 May 21;3(10):1872-9. Epub 2005 Apr 14. PMID:15889170
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