1wa0: Difference between revisions
New page: left|200px<br /> <applet load="1wa0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wa0, resolution 1.60Å" /> '''CRYSTAL STRUCTURE O... |
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==About this Structure== | ==About this Structure== | ||
1WA0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | 1WA0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans]] with CU, ZN, SO4, PG4 and MES as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WA0 OCA]]. | ||
==Reference== | ==Reference== | ||
Insights into redox partner interactions and substrate binding in nitrite reductase from Alcaligenes xylosoxidans: crystal structures of the Trp138His and His313Gln mutants., Barrett ML, Harris RL, Antonyuk S, Hough MA, Ellis MJ, Sawers G, Eady RR, Hasnain SS, Biochemistry. 2004 Dec 28;43(51):16311-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15610025 15610025] | Insights into redox partner interactions and substrate binding in nitrite reductase from Alcaligenes xylosoxidans: crystal structures of the Trp138His and His313Gln mutants., Barrett ML, Harris RL, Antonyuk S, Hough MA, Ellis MJ, Sawers G, Eady RR, Hasnain SS, Biochemistry. 2004 Dec 28;43(51):16311-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15610025 15610025] | ||
[[Category: | [[Category: Achromobacter xylosoxidans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Antonyuk, S.V.]] | [[Category: Antonyuk, S.V.]] | ||
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[[Category: w138h mutant]] | [[Category: w138h mutant]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:57:07 2007'' | ||
Revision as of 14:52, 30 October 2007
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CRYSTAL STRUCTURE OF W138H MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE
OverviewOverview
Dissimilatory nitrite reductase catalyses the reduction of nitrite to, nitric oxide within the key biological process of denitrification. We, present biochemical and structural results on two key mutants, one, postulated to be important for the interaction with the partner protein, and the other for substrate entry. Trp138, adjacent to one of the type-1, Cu ligands, is one of the residues surrounding a small depression, speculated to be important in complex formation with the physiological, redox partners, azurin I and II. Our data reveal that the Trp138His mutant, is fully active using methyl viologen as an artificial electron donor, but, there is a large decrease in activity using azurin I. These observations, together with its crystal structure at a high resolution of 1.6 A confirm, ... [(full description)]
About this StructureAbout this Structure
1WA0 is a [Single protein] structure of sequence from [Achromobacter xylosoxidans] with CU, ZN, SO4, PG4 and MES as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Insights into redox partner interactions and substrate binding in nitrite reductase from Alcaligenes xylosoxidans: crystal structures of the Trp138His and His313Gln mutants., Barrett ML, Harris RL, Antonyuk S, Hough MA, Ellis MJ, Sawers G, Eady RR, Hasnain SS, Biochemistry. 2004 Dec 28;43(51):16311-9. PMID:15610025
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