1eik: Difference between revisions
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'''SOLUTION STRUCTURE OF RNA POLYMERASE SUBUNIT RPB5 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM'''<br /> | '''SOLUTION STRUCTURE OF RNA POLYMERASE SUBUNIT RPB5 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM'''<br /> | ||
==Overview== | ==Overview== | ||
RPB5 is an essential subunit of eukaryotic and archaeal RNA polymerases. | RPB5 is an essential subunit of eukaryotic and archaeal RNA polymerases. It is a proposed target for transcription activator proteins in eukaryotes, but the mechanism of interaction is not known. We have determined the solution structure of the RPB5 subunit from the thermophilic archeon, Methanobacterium thermoautotrophicum. MtRBP5 contains a four-stranded beta-sheet platform supporting two alpha-helices, one on each side of the beta-sheet, resulting in an overall mushroom shape that does not appear to have any structural homologues in the structural database. The position and conservation of charged surface residues suggests possible modes of interaction with other proteins, as well as a rationale for the thermal stability of this protein. | ||
==About this Structure== | ==About this Structure== | ||
1EIK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | 1EIK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIK OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Methanothermobacter thermautotrophicus]] | [[Category: Methanothermobacter thermautotrophicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Arrowsmith, C | [[Category: Arrowsmith, C H.]] | ||
[[Category: Booth, V.]] | [[Category: Booth, V.]] | ||
[[Category: Dharamsi, A.]] | [[Category: Dharamsi, A.]] | ||
[[Category: Edwards, A | [[Category: Edwards, A M.]] | ||
[[Category: Engel, A.]] | [[Category: Engel, A.]] | ||
[[Category: NESG, Northeast | [[Category: NESG, Northeast Structural Genomics Consortium.]] | ||
[[Category: Yee, A.]] | [[Category: Yee, A.]] | ||
[[Category: nesg]] | [[Category: nesg]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:09 2008'' | ||
Revision as of 13:28, 21 February 2008
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SOLUTION STRUCTURE OF RNA POLYMERASE SUBUNIT RPB5 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM
OverviewOverview
RPB5 is an essential subunit of eukaryotic and archaeal RNA polymerases. It is a proposed target for transcription activator proteins in eukaryotes, but the mechanism of interaction is not known. We have determined the solution structure of the RPB5 subunit from the thermophilic archeon, Methanobacterium thermoautotrophicum. MtRBP5 contains a four-stranded beta-sheet platform supporting two alpha-helices, one on each side of the beta-sheet, resulting in an overall mushroom shape that does not appear to have any structural homologues in the structural database. The position and conservation of charged surface residues suggests possible modes of interaction with other proteins, as well as a rationale for the thermal stability of this protein.
About this StructureAbout this Structure
1EIK is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum., Yee A, Booth V, Dharamsi A, Engel A, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6311-5. PMID:10841538
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- DNA-directed RNA polymerase
- Methanothermobacter thermautotrophicus
- Single protein
- Arrowsmith, C H.
- Booth, V.
- Dharamsi, A.
- Edwards, A M.
- Engel, A.
- NESG, Northeast Structural Genomics Consortium.
- Yee, A.
- Nesg
- Northeast structural genomics consortium
- Ocsp
- Protein structure initiative
- Psi
- Rna polymerase subunit
- Rpb5
- Rpbh
- Structural genomics